Effects Of Baicalin On Rabbit Lens Epithelial Cell Proliferation Inhibition And NF-κB

Aim To investigate the effects of Baicalin on proliferation inhibition and apoptosis induction in rabbit lens epithelial cell and to explore its underlying mechanisms.Methods rabbit lens epithelial cell were exposed to baicalin at diferent dosages and its proliferation inhibition was detected by MTT assay.The ability of baicalin to induce lens epithelial cell apoptosis was examined by Annexin V-FITC/PI double staining analysis.The mRNA expressions of NF-κB P65 were detected by RT-PCR.Results Baicalin remarkably inhibited the rabbit lens epithelial cell proliferation, with an IC50 value of 46.32μg/m1 .Apoptosis was remarkably induced by baicalin in a dose-dependent manner,its earlier stages were detected by annexin V-FITC/PI double staining analysis.RT-PCR showed that the mRNA expressions of NF-κB p65 in rabbit lens epithelial cell decreased in a dose-dependent manner.Conclusion Baicalin efficiently induces proliferation inhibition and apoptosis in rabbit lens epithelial cell,which may be related with the down-regulation of NF-κB.