The Effect Of Mat On Rabbit Lens Epithelial Cells In Vitro

Objectives:To study the effects of Matrine(Mat) on the proliferation of rabbit lens epithelial cells (RLECs) in vitro and to provide a new clue and theory basis for developing a drug of preventing and curing posterior capsular opacification(PCO) in the future.MethodsrRLECs were primary culture. After the third generation of RLECs were incubated for 24 hours, we added different concentrations of Mat and 0.02mg/ml MMC for different period. The cell proliferation of RLECs was determined by MTT assay and the cell apoptosis of RLECs was detected by flow cytometry(FCM). Results: 1.After the RLECs were incubated for 24 hours with different concentrations of Mat (0.1, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2, 1.4, 1.6 mg/ml), the inhibitory rates of which were 5.72%, 12.50%, 19.43%, 25.94%, 34.37%, 42.49%, 54.56%, 62.93%, 70.39% respectively through MTT assay. There were significant differences between experimental groups in which the RLECS were disposed with different concentrations of Mat and control group disposed with PBS(P<0.05), as well as among experimental groups (P<0.05). The inhibitory rates of RLECs increased with the raise of the Mat concentrations. Then, we observed the inhibitory effects of Mat on RLECs, which were exposed to high(1.5 mg/ml), middle(1.0 mg/ml), low(0.5 mg/ml) concentrations for 12, 24, 36, 48, 72 hours respectively. The inhibitory rates of RLECs by 0.5 mg/ml of Mat were 11.64%, 20.47%, 35.42%, 45.82%, 52.04% for incubation time of 12, 24, 36, 48, 72 hours respectively, they are 27.37%, 41.01%, 49.99%, 63.26%, 70.80% by 1.0 mg/ml of Mat, and 46.98%, 64.36%, 73.09%, 81.64%, 89.32% by 1.5 mg/ml of Mat. When the concentrations were identical, significant differences were seen between experimental groups in which the RLECS were exposed to different incubation time and control group exposed to 0 hour(P<0.05), so were among experimental groups (P<0.05). The inhibitory rates of RLECs increased with both the raise of the Mat concentrations and the prolongation of time. 2.The RLECs were...