Analysis Of The Clinicopathologic, Immunohistochemistry And Mutation Of Gene Exons In 74 Cases Of Gastrointestinal Stromal Tumor In Shenzhen

Objective To explore the characteristics of clinicopathologic,immunohistochemistry and gene exons mutation of gastrointestinal stromal tumor in Shenzhen.Methods Analyze of retrospectively clinicopathological feature of 74 surgical cases of GIST in Shenzhen. The expression levels of CD117,CD34,SMA and S-100 were detected by immunohistochemical method.Mutation of c-kit (exon 9,11,13,17) and PDGFRA gene (exon 12,18) in 13 cases of GIST whose diameters are more than 5cm were examined by PCR amplification and direct sequencing.Results Positive rates of the CD117,CD34,SMA and s-100 were 83.8% (62/74),81.1% (60/74),31.1%(23/74) and 5.4%(4/74) respectively.12 cases with mutation were detected in 13 cases of GIST which diameters are more than 5cm, including 8 cases(61.5%)with c-kit exon 11 mutations,2 cases(15.4%)with c-kit exon 9 mutations,1 case (7.7)with c-kit exon 13 mutations,1 case(7.7%)with PDGFRA exon 18 mutations.Conclusions CD117 and CD34 are could be the diagnosable marker of GIST because they are quite sensitive. C-kit exon 11 may play an more important role in predicting the prognosis for GIST,since it is more obvious in high risk GIST than others. The characteristics of clinic, pathology, immunohistochemistry and gene exons mutation of of gastrointestinal stromal tumor in Shenzhen were consistent with reports of domestic and international bulk.