Signification Of PDGFRα Gene Mutations And Expression In Gastrointestinal Stromal Tumour

Researches have demonstrated that C-Kit gene mutation and the protein expression were the major reason of the tumorigenesis of gastrointestinal stromal tumour (GIST). But the relationship between platelet-derived growth factor receptor-alpha (PDGFRa) gene mutation, the protein expression and GIST were very close. In order to demonstrate signification of PDGFRa gene mutation and the protein expression in GIST, we use PCR-SSCP, Immunohistochemical and Western-blotting to detect PDGFRa and C-Kit gene mutation and protein expression in GIST. The relationship between mutations, clinicopathological features and prognostic factors will be analyzed by x test and survival analysis. Conclusion: PDGFRa gene mutation rate is 9.6%, most are spindle histology and gastric origin, besides tumors with C-Kit mutation aren't be examined PDGFRa mutation;The GISTs with PDGFRa mutation have the better prognosis;PDGFRa protein expression significantly associated with corresponding gene mutation;the 5 years survival of patients was 69.81%, the tumor size≥ 10cm, mitotic counts >15/50HPF and high-risk grade is correlated with poor prognosis. And risk grade may be the best simply prognostic estimated criterion.