| ObjectiveUsing glycol and ammonium chloride SD rats induced by male animal model of calcium oxalate stone; Making potassium citrate to discuss it influence and mechanism in SD rats of calcium oxalate stone. Therefore it provide material and ideas for prevention of calcium oxalate stone.Methods1. Health male sprague-dawley(SD)rats were given content of 1% ethylene glycol and 1% ammonia chloride water to establish the rat renal calcium oxalate calculi model. 2. Twenty-four of health SD male rats randomly were divided into three groups(N=8 each). group A (normal control group); group B(group stone formation); group C (group potassium citrate supplementatio intervention). 3. After experiment two week, the concentration of the 24h urinaty excretion Ox,Ca2+ ; serum of BUN,Cr,Ca2+,P,MDA,SOD and the content of renal tissue MDA,SOD were detected in each of group rats; All kidneys were removed and CaOx crystals deposit and tubular dilatation in the kidney were observed under light microscopy (HE-staining); Immunohistochemistry method was used to assess the protein expression of OPN in rat renal tissue of every groups.Results1. Urine test index results: 24h urine concentration of Ox and Ca2+ show that group A significantly below group B,C (P﹤0.05) and group C significantly below group B (P﹤0.05). 2. Bloodtest index results: Serum concentration of BUN, Cr show that group A significantly below group B,C (P﹤0.05); Compared with group B, the group C serum concentration of BUN were decreased, but it is not statistically significant differences (P=0.076, p﹥0.05) and serum concentration of Cr were significantly decreased (P﹤0.05). Each Ca2 +,P concentration of blood show that they are not statistically significant differences (P﹥0.05); Compared with group A, the enzyme activity of superoxide dismutase(SOD) were significantly decreased and the group B concentration of malondialdehyde(MDA) were significantly increased in serum (P<0.05); Compared with group B, the group C activity of SOD were significantly increased and the enzyme concentration of MDA were significantly decreased in serum (P<0.05). Compared with group A, the group C activity of SOD were increased, but it is not statistically significant differences (P=0.064, p﹥0.05). Meanwhile, the enzyme concentration of MDA were significantly decreased in serum (P<0.05). 3. Renal tissue index results: Compared with group A, the enzyme activity of SOD were significantly decreased and the group B concentration of MDA were significantly increased in serum (P<0.05), Compared with group B, the group C activity of SOD were significantly increased and the enzyme concentration of MDA were significantly decreased in serum (P<0.05). Compared with group A, the group C activity of SOD were significantly increased and the enzyme concentration of MDA were significantly decreased in serum (P<0.05). 4. HE dyeing and stone crystal score results: group A Renal tubular is normal; In group B renal tubules were seen cavity of calcium oxalate and obvious expansion; In group C renal tubules were seen a cavity of calcium oxalate, without obvious expansion; stone crystal score results show that group C Stone crystals more obvious reduction than group B (P<0.05). 5. OPN protein immunohistochemical results: In rats of normal control group A, main of a weak OPN staining was noted; The expression was enhaneed in the stone group B; the group C have lower OPN protein expression than the stone B.conclusions1. The method of 1%ethylene glycol plus l%ammonia chloride for two week could make renal calcium oxalate calculus mode; 2. Potassium citrate could reduce the formation of SD male rats of renal calcium oxalate crystallization; 3. The mechanism may be citrate complex with calcium binding,so that making urine concentration of Ca2+ and calcium oxalate saturation are decreasion; through the antioxidant effect, it protect renal tubular epithelium; Meanwhile, it may be inhibit the OPN expression and deposit of calcium oxalate in renal tissue so as to block the formation of renal renal calcium oxalate stone inrats potently. |
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