Expression And Clinical Significance Of Parp-1, Caspase-3 In Breast Cancer

Objectives:This research was designed to investigate the expression and relationship of PARP-1 and caspase-3 protein in normal breast, benign lesional and breast cancer, and their correlation with clincopathologic features, for provide evidence for breast cancer of diagnosis and prognostic evaluation.Methods:1 The 282 paraffin-embedded specimens were collected, including 267 cases of invasive breast cancer and 15 cases of benign lesions. 11 cases of fresh normal breast, 10 cases of fresh benign lesions and 10 cases of fresh invasive breast cancer were collected. Diagnosis of all cases, sub-type classification, according to the WHO(2003) breast tumor histologic classification.2 The expression of PARP-1 and caspase-3 was measured by immunohistochemistry SP methods in 11 cases of normal breast, 25 cases of benign lesions and 277 cases of invasive breast cancer. Analyze the relationship between the expression of PARP-1 and caspase-3 and the pathological characteristics.3 In situ hybridization method was used to examine for the expression, relation and the effect of PARP-1 mRNA in 10 cases of fresh normal breast, 10 cases of fresh benign lesions and 10 cases of fresh invasive breast cancer.Results:Immunohistochemistry results showed that1 The expression and relevance of the PARP-1 and caspase-3 protein1.1 The expression of the PARP-1 protein in breast cancer, benign breast lesions and normal breast The positive rate of the expression of PARP-1 in breast cancer (65.70%, 182/277) is higher than that in normal breast (27.27%, 3/11) and benign lesions(44.00%, 10/25), the difference has statistical significance(p=0.009, p=0.011). There is no statistical significance on positive rate of the expression of PARP-1 between normal breast and benign lesions(p>0.05).1.2 The expression of the caspase-3 protein in breast cancer, benign breast lesions and normal breastThe positive rate of the expression of caspase-3 in normal breast(81.82%, 9/11) is higher than that in breast cancer(42.24%, 117/277) and that in benign lesion, the difference has statistical significance(p=0.009, p=0.035).There was no statistical significance on positive rate of the expression of caspase-3 between benign lesions and breast cancer (p>0.05).1.3 The relation between the parameters of clinicopathological and the expression of the PARP-1 proteinThere is no statistical significance between The positive rate of the expression of PARP-1 and age, tumour size, histological classification(p>0.05). The positive rate of the expression of PARP-1 in histological gradeâ… ,â…¡,â…¢period were upward trend, the difference between group is significance(p<0.001). The positive rate of the expression of PARP-1 in the lymph node matastasis group(76.47%, 130/170) is higher than that in the no lymph node matastasis group(48.60%, 52/107), the difference has statistical significance(p<0.001).1.4 The relation between the parameters of clinicopathological and the expression of the caspase-3 proteinThere is no statistical significance between The positive rate of the expression of caspase-3 and age, tumour size, histological classification, histological grade (p>0.05).1.5 The correlativity of PARP-1 and caspase-3 protein expression in breast cancerThe positive rate of the expression of PARP-1 is negative correlation with caspase-3, correlation analysis showed that PARP-1 and caspase-3 in breast cancer tissues are direct correlation(r=-0.722, p<0.001).In situ hybridization results showed thatPARP-1 mRNA expression in breast cancer is higher than the normal group and benign breast lesions, the difference has statistically significance(p=0.007, p=0.025).Conclusions:1 The expression of PARP-1 in breast cancer is higher than that in normal breast and in benign lesions.2 The expression of caspase-3 in breast cancer is lower than that in normal breast and in benign lesions.3 There is statistical significance between the positive rate of the expression of PARP-1 and histological grade, lymph node matastasis.4 The positive rate of the expression of PARP-1 is negative correlation with caspase-3 of in breast cancer. Increased expression of PARP-1 may be related to the malignant biological behavior of breast cancer. PARP-1 and caspase-3 were conduced to the judgement and prognosis of breast cancer.