The Therapeutic Effects Of VEGF Receptor Tyrosine Kinases Inhibitors On Hypertrophic Scar In Rabbit Ear

Objective: To investigate the possibility of pathologic scar treated in VEGF Receptor Tyrosine Kinases,by observing the effects of expression of collagen type I in hyperplastic scar and neogenesis,capillary of Pathologic scars treated in VEGF Receptor Tyrosine Kinases,through establishing models of Hypertrophic scar in rabbit ear and injecting VEGF Receptor Tyrosine Kinases.Methods: 16 New Zealand white rabbits were used to estabilish the model of hypertrophic scar in rabbit ears,which was randomly divided into 3groups: Dynamic model group. using general and morphological observation of wound healing and scar formation; On days 6,12,16,22,30,42,56after modeling,the change of texture and healing of wound surface were observed.Experiment group (VEGF Receptor Tyrosine Kinases injected) Model group(NS injected ).10 days after surgery,the hypertrophic scar were injected intralesionally with VEGF Receptor Tyrosine Kinases II and NS.2 days injection of once for every wound,a total of six times.The scars were harvested 8days after the last injection for measurement of scar elevation index(SEI).The groups are evaluated through pathological sections HE Masson staining, The change of CD34,VEGF,bFGF were addressed by immunohistochemistry.The expression of type I pro-collagen mRNA were assessed by reverse transcription polymerase chain reaction(RT-PCR).Results: General observation: The epithelization time of the wounds is in 10-15days.18 days after epitheliazation,the high of excess dermal scarring is as 3 times high as original ventol skin,color is pink,the blood vessel is abundant;The scars begin to soften in about 50 days,the excess scarring can last nearly90-100days;The color and luster of scar after treating in VEGF Receptor Tyrosine Kinases become light,the thickness is reduced.and become smooth.The area of scar in experiment groups is obviously less than model groups(p<0.01).Pathological section: the counts of capillaries in experiment groups are obviously less than control groups(p<0.01);On the contrary,blood capillary and fibrocyte hyperplasia,the collagen deposits in a large amout.The expression of VEGF and bFGF in hypertrophic scar on ears of rabbits were also studied by immunohistochemistry staining at the thirtieth day after VEGF Receptor Tyrosine Kinases being injected.The results show that the expression of VEGF and bFGF were clearly depressed(p<0.05).The expression of type I pro-collagen mRNA decreased in model group,which showed statistical difference compared with Experiment groups.(P<0.05).Conclusion: The results of animal experiments suggest that VEGF Receptor Tyr- osine Kinases II is able to inhibit the proliferation process of hypertrophic scar in rab- bit ears and to remarkably decrease the degree of fibrosis in the scar. VEGF Receptor Tyrosine Kinases would become a new clinical treatment of hypertrophic in the fut- ure.