Mucopolysaccharide Polysulfate Cream Prevents And Inhibits Hypertrophic Scar Formation In A Rabbit Ear Model

Background Hypertrophic scar（HS）is a unique abnormal skin disease of human beings,which is characterized by excessive collagen deposition in the dermis or subcutaneous tissue secondary to trauma or surgical injury.It usually occurs within 4 to 8 weeks after wound infection,overstretched closed wound,or other traumatic skin injury.It has a rapid growth period of up to 6 months,then gradually fades within a few years,eventually leading to a flat scar with no further symptoms.In recent years,because of its cheap price and easy to use,mucopolysaccharide polysulfate cream has been gradually used by dermatologists to prevent and inhibit the formation of hypertrophic scar.In order to further explore the relationship of mucopolysaccharide polysulfate cream in the prevention of hypertrophic scar,the rabbit ear scar model was selected and the wound was treated with mucopolysaccharide polysulfate cream.The changes of scar hyperplasia,inflammatory reaction and collagen content in rabbit ears were observed.To explore the role and mechanism of polysulfonate mucopolysaccharide cream in hypertrophic scar.Objective To establish an animal model of hypertrophic scar in rabbit ears and to study the mechanism of mucopolysaccharide polysulfate cream in preventing and inhibiting scar formation.Methods The rabbit ear hypertrophic scar model was established,and 16 New Zealand white rabbits were selected to establish a round full-thickness wound with a diameter of 6mm in both ears.The scar of the left ear of the rabbit was selected as the experimental group,and the scar of the right ear of the rabbit was selected as the matrix control group.On the 14 th day after operation,the experimental group began to apply mucopolysaccharide polysulfate cream externally,using one fingertip as the dosage of one rabbit ear,twice a day.It was used continuously for 6 weeks.The control group was smeared with cream base in the same way.According to the sampling time,96 wounds were divided into three groups.The samples were collected at 14 days after operation（before treatment）,28 days（14 days after treatment）and 56 days（42 days after treatment）.The corresponding indexes of scar tissue were detected by HE staining,Masson staining and immunohistochemistry.Through the comparison between the two groups and different time within the group,the effect of mucopolysaccharide polysulfate cream on scar was evaluated.Results Comparing the scar thickness between the experimental group and the control group,there was no significant difference in scar thickness between the control group and the experimental group on the 28 th day after operation（P = 0.484）.On the 56 th day after operation,there was significant difference in the scar thickness between the control group and the experimental group（P < 0.010）.Comparing the collagen density between the experimental group and the control group,on the 56 th day after operation,there was significant difference in the collagen density between the control group and the experimental group（P < 0.050）.The differences of collagen fiber density at different time in the experimental group were compared: 1 there was significant difference in collagen fiber density between 28 days after operation and 14 days after operation（P < 0.05）.2 there was significant difference in collagen fiber density between 56 days and 14 days after operation（P < 0.05）.Conclusion The experimental results show that the local application of mucopolysaccharide polysulfate cream is effective in preventing and inhibiting the proliferation of hypertrophic scar.It is mainly manifested in the inhibition of hypertrophic scar thickness,collagen fiber density and inflammatory reaction.