Effects Of Sports Training On Spatial Learning And Memory Of Rats And On The Related Genes In Brain

Objective: Rat's different intensity of swimming exercise training on spatial learning and memory ability and brain-related genes c-fos, c-jun, BDNF mRNA expression.Methods: Selected of 48 male SD rats, the 3-month-old ,fed adaptive 1 week. To experimental animals were randomly divided into three groups, each group have 16 rats.Those were in the control group, the aerobic exercise group and exercise fatigue group. And the three groups of rats were divided into A, B two groups ,each group has 8 rats .Aerobic exercise group and the fatigue of the campaign group of rats in the first week training adaptation for 3 times, each 30 minutes. 8 weeks beginning of the next formal swimming training, 6 times a week swimming training. Aerobic exercise group swimming every 60min; campaign fatigue rats to swim every time of 180min, or appear in 180min movement in a row, could not on surface, limbs uncoordinated, the emergence of the phenomenon of choking water .When the fatigue is still time to complete the training. After the completion of the model, the first group A measure body weight, and then A group using the Morris water maze behavioral indicators for the test. Which took place navigation test 3 days, hours a day, the afternoon of a training program, each rat in a procedure within Training 4. Every time from a fixed location for the rats into the water wall, a computer monitor and record the water rat from the beginning to search for and find a platform to climb up the time needed for each quadrant of the activities of the distance and time, the initial angle, and so on . Experiments in search of space navigation positioning after the dismantling of the experimental platform, and then from the original location of the wall into the water for rats, rats with record 3 minutes to cross the original platform, the number of times. Group B in the rat model was completed immediately put to death out of the brain hippocampus, RT-PCR for detection of hippocampal c-fos, c-jun, BDNF mRNA expression.Results:1. Rats in each group after 8 weeks of feeding and swimming training, body weight significantly increase the performance of the control group> aerobic training group> fatigue group2. With the rats to learn to extend the number of times, reduce the incubation period, the average incubation period of the control group dropped quickly and maintain relatively stable; fatigue campaign group the average incubation period of slow decline and volatile; and aerobic exercise group The average incubation period is between performance and fatigue in the control group between.3. Three groups of rats by using a random-search approach, and linear trend-less edge-search methods are rarely used in the control group of rats. Fatigue group in the four ways to use the trend-line and search a relatively small way. Rats in the control group used less-edge search mode.4. 3 rats across the platform with the number 3, number of samples were found to be 22 q test prompted comparisons with the control group aerobic exercise group did not differ significantly (Q = 1.2869, P> 0.05), and fatigue in the control group the difference between the exercise group significantly (Q = 3.9679, P <0.05), aerobic exercise group and campaign fatigue group does not differ significantly (Q = 2.6810, P> 0.05). 5. 8 weeks after swimming, aerobics and sports fatigue rat hippocampus c-fos, c-jun, BDNFmRNA expression than those in the control group there is a significant increase (P <0.05).6. Fatigue exercise group TLR4mRNA expression within the hippocampus compared with control group increased significantly (P <0.05). The aerobic exercise group were not.Conclusion : (A) fatigue movement and control groups compared to 8 weeks of swimming exercise training can make the rats in the Morris water maze in a significant decline in performance. This suggests that excessive exercise training can reduce the rat's spatial cognition and memory, from one side and show the fatigue of the campaign on mental health damage.(B) of the aerobic exercise group, the Movement of fatigue and control groups compared to 8 weeks of swimming training in the hippocampus, to varying degrees, caused by c-fos, c-jun, BDNFmRNA expression increased significantly. Thus suggest that exercise training can promote the expression of these genes in the brain.(C) TLR4 expression may lead to a large number of apoptosis in rat hippocampus...