Study On Relationship Between The Expression Of P27 And P53 In Epithelial Ovarian Tumors

Objective: Ovarian cancer is one of the most common women's reproductive system cancers. It has been confirmed that ovarian cancer is associated with a variety of proto-oncogenes and tumor suppressor genes variation. such as Ras gene, erbB-2 gene, P16 gene. Among them, the tumor suppressor gene P53 and its family members, and P21 and its family members P27 that is the candidate related to ovarian cancer. Of the total, tumor suppressor gene P53 is the most deedly gene to study and research now. Is generally think that there have affinity between P27 gene and ovarian cancer.As the generally tumor suppressor gene, P53 concerned with the important biological function such as Cell Cycle control, DNA copy,cell differentiation apoptosis. The mutation and lose of P53 is the considerable sign of oncogene. Mutational P53 lose the action to restrain carcinoma, so it lose monitor function to the cells in G1 Phase, and it can not make the damaged cells apoptosis. Of mutant P53 the stability raise, half life period lengthen, and gathreing in cell nucleus, so we can check out P53 adopt the immuno histochemisty. We can say that mutant P53 albume exist when antibody of P53 masccline, and then it sidelight that P53 gene mutate. P27 is one of the most important member in KlP family, both KlP family and INK4 family are the two familys in CKIS. P27 can inhibit activity of many cyclin and CDK compound, negative regulate cell cycle,participate to mediate rejection capability aignal transduction ecto-cell and to accommodate tumor resistance, and P27 have the actions to advance differentiate and to add adhesiveness betweencells. Accordingly, conduct and action a candidate tumor suppressor gene, lucubrating expression of P27 in ovarian neoplasms become popular gradually. The abnormal expression of P27 and P53 can promote the occurrence and development of epithelial ovarian neoplasm. However, there is no coverage at home and abroad on the relationship between P27and P53. This study will explore both in epithelial ovarian neoplasm'expression and their relationship and will provide areference indicators for ovarian cancer's diagnosis and treatment. Meanwhile, detecting P27and P53 genes in primary epithelial ovarian neoplasm and its relationship with clinicopathological features. So that to explore the efection both in occurrence and development of primary epithelial ovarian neoplasm and the prognosis, clinical value and relevance of them.Methods: 120 cases of ovarian tumor specimens were collected (during 2008-2009). In these, 25 cases of benign ovarian tumor; 18 cases of borderline ovarian epithelial neoplasm; 57 cases of malignant ovarian tumor (39 cases of serous cystadenocarcinoma; 9 cases of mucinous cystadenocarcinoma; 9 cases of endometrial carcinoma). Taking 20 cases of normal ovarian tissue as normal control which resectied during the same period from hysteromyoma tumor resection or surgery for non-neoplastic reasons. All patients were aged from 35 to 55 years old. All tissues were 10% formalin fixed and paraffin- embedded solution. All specimens were confirmed by pathology, neither chemotherapy nor radiotherapy treated before surgery. Using immunohistoch- emical S-P method for detection. Dealing data with SPSS13.0 package. Analyzing results byχ2 test and spearman rank correlation. The results are significant difference when P<0.05.Results: 1 The positive expression rate of p27 in normal ovarian tissue, ovarian benign, borderline and malignant tumors decrease gradually, the positive expression rate is 90.00%, 80.00%, 55.56% and 36.84% in four groups. P27 protein expression in malignant group significantly lower than the normal group and the benign group , The difference has statistical significance (p<0.05). And there is significant difference between the benign group and the normal group (p<0.05). But there is no significant difference between the normal group and benign group, the borderline group and the benign group, the malignant group and borderline group (p>0.05).2 In the epithelial ovarian cancer, there is no significant difference of P27 protein expression level among different ages, pathological type and lymph node metastasis. But the P27 protein expression level concerned with the clinical type and pathological stage, the difference has statistical significance (p<0.05). The expression level of P27 in poorly differentiated group is significantly lower than high differentiation group, the positive rate of P27 inⅢ-Ⅳperiod is significantly lower thanⅠ-Ⅱperiod.So the difference has statistical significance (p<0.05).3 There is no P53 expression in nomal ovarian tissue, but the positive expression rate of P53 in ovarian benign, borderline tissue and malignant tumors increased gradually, the positive expression rate is 12.00%, 16.67% and 63.16%. P53 protein expression in malignant group significantly higher than the nomal ovarian tissue, ovarian benign tissue and borderline group, the difference has statistical significance (p<0.05).4 In the epithelial ovarian cancer, there is no significant difference of P53 protein expression level among different ages, pathological type and lymph node metastasis. But the P27 protein expression level concerned with the clinical type and pathological stage, the difference has statistical significance (p<0.05). The expression level of P53 in poorly differentiated group is significantly higher than high differentiation group, the positive rate of P53 inⅢ-Ⅳperiod is significantly higher thanⅠ-Ⅱperiod. So the difference has statistical significance (p<0.05).5 In the epithelial ovarian cancer, negative correlation exists between P27 and P53 expression (Rs = -0.397, P<0.05).Conclusions: 1 In the developing process of transformation from benign to malignant epithelial ovarian tumor, the trend of expression level of P27 is decreased, and its expression is higher in malignant tumor than in benign tissue, borderline tumor and nomal ovarian tissue, which showes that the trend of expression level of P27 is decreased maybe playing a promoting role in transformation and development of some kind from benign tumor to malignant tumor.2 There is some relation between the P27 expression and the tissue differentiation of ovarian cancer. Expression level of P27 decreases from high differentiation group to poorly differentiated group. And There is relation between the P27 expression and the clinical type of ovarian cancer. Expression level of P27 decreases fromⅠ-Ⅱperiod toⅢ-Ⅳperiod, which shows that it maybe used as a sign of malignant epithelial ovarian cancer and the pathological grade meaning a poor prognosis.3 P53 protein showes no expression in nomal tissue, but positive expression in benign tumor, borderline tissue and epithelial ovarian tumor. In epithelial ovarian cancer, P53 protein expression increases, which related to the occurrence of ovarian cancer.4 P53 protein expression is closely related to the clinical type, pathological stage in epithelial ovarian cancer, The expression level of P53 in poorly differentiated group is significantly higher than high differentiation group, and inⅢ-Ⅳperiod is significantly higher thanⅠ-Ⅱperiod, showing that P53 gene's abnormal expression playing important roles in the development of epithelial ovarian cancer.5 In epithelial ovarian cancer, expression of P27 and P53 existes a negative correlation, which prompts that P27 and P53's abnormal expression playing a interaction in the occurrence and development of epithelial ovarian cancer.