Research The Hcmv-pp65 And VCAM-1 In Ischemic Cerebrovascular Disease Patients With Different Type Of Atherosclerotic Plaques

Objective: Artherosclerosis is the main pathological basis of cerebral infarction. Studies have shown that more than half of ischemic cerebrovascular disease patients associated with artherosclerosis. The incidence rate of ischemic cerebrovascular disease tend to increase every year,especially among young people. Therefore, to find the cause of atherosclerosis and carry out targeted prevention is an urgent need to address the clinical problems.In recent years,a number of studies have shown that microbial infection,especially human Cytomegal ovirus,HCMV infection and activation related to atherosclerosis. HCMV active infection caused by inflammation and innune respons in AS and plays an important role in the occurrence and development The main of this article is to investigate the relationship between carotid artery artherosclerosis and activated infection of HCMV and VCAM-1 .Methods:1 The investigation subjects were 84 selected cases which was complied with the conditions, all of the cases conduct ultrasound of arteria carotid, HCMV-PP65 of peripheral blood leucocyte,VCAM-1.Compare the differences of HCMV-PP65,VCAM-1 in the patients with different kinds of plaques, further to compare the differences of VCAM-1between PP65 positive and negative paitents.2 Ultrasound examination method: Use color Doppler ultrasonograph of Siemens IU-22 producted by Germany. With linear array detecting head, frequency of waves is 5～12MHZ.With dorsal position, detecte from originate of arteria carotis to earteria carotis interna when entered the skull.Examine both of the arteria carotis use consecutive T-sect ,longitudinal plane 2D gray-scale and frequency spectrum ultrasonic examination by Doppler's method. Review and record the IMT,mainly observe whether exist atherosclerotic plaque or not, the position, size, resonance ,surface characteristic of atherosclerotic plaque.3 Detect HCMV-PP65 antigen in peripheral blood leucocyte use the method of immunohistochemical; Assaying VCAM-1 in peripheral blood use the metod of biocatalyst connected immune adsorption double antibody sandwich.4 The various plaques were grouped into 6 types on the basis of the course of disease development, kinds of pathologic changes and Hennerici classification. For the statistical convenience, the patients with soft and flat plaque were included into instability plaque group, the patients with endometrial hyperplasia and hard plaque were classified into stability plaque group.Results:1 Different plaques have different HCMV-PP65 postive rate, in the instable plaques is 56.76%,it is obviously higher than the stable ones(30.30%) (P<0.05).2 The average contents of VCAM-1 are 363.61 ng/ml in HCMV-PP65 positive group and 305.92 ng/ml in negtive one. There is a significant difference between the contents of the two groups (P<0.05).3 The average content of VCAM-1 in plaque group is 352.78 ng/ml, with 219.99 ng/ml in no-plaque group, and the level of VCAM-1 of the former is higher than that of the latter (P<0.05). The average content of VCAM-1in instable-plaque group is 380.66 ng/ml, with 321.51 ng/ml in stable-plaque group, and the level of VCAM-1 of the former is also higher than that of the latter (P<0.05). The data suggest the expression of VCAM-1 differs along with the type of plaques.4 In the positive cases, the average content of VCAM-1 in plaque group is 383.79 ng/ml, with 238.48 ng/ml in no-plaque group, and the level of VCAM-1 of the former is higher than that of the latter (P<0.05). The average content of VCAM-1 in instable-plaque group is 403.72 ng/ml, with 341.95 ng/ml in stable-plaque group, and the level of VCAM-1 of the fomer is also higher than that of the latter (P<0.05). After infection of HCMV, there is a positive correlation between the expression of VCAM-1 and instability of plaque.Conclusions: In the instable plaques HCMV-PP65 postive rate is obviously higher than the stable ones. The average contents of VCAM-1 in HCMV-PP65 positive group is higher than negtive groups. In the positive cases, The average content of VCAM-1 in instable-plaque group is higher than stable-plaque group,which direction that the HCMV active infection plays an important role in the AS, HCMV active infection may promote the expression of VCAM-1, and there is a tendency to promote plaque instability . VCAM-1 has positive correlate with HCMV and AS, and inflammatory reaction of infection of HCMV plays an important role in atherogenesis.