| Objective:1,This subject aims to observe the effect of Astragalus infection (AI)on the vascular endothelial function,cardiac function and the clinical symptoms for the patients with acute myocardial infarction(AMI),2,To research the possible mechanism for stimulating angiogenesis of AI.Methods:First, the clinical research:The patients with acute myocardial infarction were collected from Jiangsu Province hospital of Chinese medicine who were diagnosed clearly and accepted the treatments during December,2008 to December,2009. They were randomly divided into the AI group (20 cases) and the control group(20 cases). The latter was treated with conventional treatments, while the former was treated with Astragalus infection besides conventional treatments. The two groups did't show significant differences in the following aspects:age, sex, body weight index, dangerous factors, cardiac function, the type of AMI and so on. The treatment course experienced 12±2 days. Before and after the treatment, we recorded the values of NO, ET, SOD, the graduation of cardiac function, and evaluated scores of Chinese medcine symptoms.Second,In order to evaluate the effects of Astragalus on stimulating angiogenesis, this study used the following experiences:cell's multiplication, migration, and vascularization. And by molecular biological technology, we investigated the effect of Astragalus on the expression of VEGF.The datas were analyzed by SPSS 13.0, and P< 0.05 has significant difference.Results:1st,the clinical research:(1) After the treatment, all over of the patients were better than before accroding to the integral table of Chinese medcine symptoms, the improvement of the AI group was more obvious than the control group (P<0.05).(2) According to the integral table of Chinese medcine symptoms.the patients of the two groups were better than before, the improvement of the AI group was more obvious than the control group in the following aspects((P<0.05)):Chest pain's duration, fatigue, Spontaneous sweating, the total scores.(3) the graduation of cardiac function. the improvement of the AI group was obvious and the difference had statistical significance (P<0.05). the control group improved than before, but didn't have statistical significance (P>0.05).(4) NO, ET, SOD:after the treatment,NO, SOD, ET of the patients in the AI group were better than before, the differences had statistical significance (P<0.05), while the control group did't show obvious improvement; Compared with the two groups, the differences of NOdid't have statistical significance (P>0.05), the differences of SOD,ET had statistical significance (P<0.05).2nd, the experimental study:(1) Through the cells'multiplication experiment, we discovered that Astragalus can promote the endothelial cells'multiplication of the big mouses'thoracic aorta obviously, compared with the blank group, the cells'vigor increased 90.22%, the difference had remarkable statistical significance (P<0.01).(2) The cells'migration experimental result indicated that Astragalus can promote the endothelial cells'migration of the big mouses' thoracic aorta obviously, compared with the blank group, the migration strength increased 211.43%, the difference had remarkable statistical significance (P<0.01).(3) Through the cells'Vascularization experiment, we discovered that Astragalus can promote the endothelial cells'Vascularization of the big mouses'thoracic aorta obviously, compared with the blank group, the number of Vascularization is 4.26 times,the difference had remarkable statistical significance (P<0.01).(4) Through the Western Blot experiment, we discovered that Astragalus can promote the expression of VEGF of the endothelial cells of the big mouses'thoracic aorta, compared with the blank group, it is higher by 86.8%, the difference had remarkable statistical significance (P<0.01).Conclusions:(1) The Astragalus infection can improve the clinical symptoms of the patients with AMI effectively, especially on the symptoms of Qi deficiency.(2) The Astragalus infection is effective for the improvement of the cardiac function of the patients with AMI. It is beneficial for the long-term prognosis.(3) The Astragalus infection can improve the function of vascular endothelial cell, eliminate the ability of cells'oxidative stress.(4) Astragalus can promote the endothelial cells'multiplication, migration,Vascularization, the expression of VEGF...
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