| Objective:In recent years, studies have shown that the generation of neuropathic pain is closely related to the and spinal cord dorsal horn neurons in sensitization,spinal cord dorsal horn neurons in long-term potentiation of synaptic plasticity.It has important significance to study acupuncture on treatment of neuropathic pain and neuropathic pain mechanism,long-term potentiation,and whether acupuncture can inhibit the production of long-term potentiation. This study is sciatic nerve injury in rat model of selective branches to observe the power for the pain threshold of neuropathic pain in rats and spinal cord dorsal horn synaptic transmission and long-term potentiation of the inhibitory effect in order to further explore the mechanism of acupuncture analgesia.Methods:Healthy adult male SD rats after screening, eligible rats were randomly divided into sham operation group, model group, and electro acupuncture intervention group. Selective Preparation of sciatic nerve branch injury neuropathic pain model (SNI) model. The model group observed 14 days and observed SNI before surgery, the next day, the 7th day, the first 14 days of determination of the mechanical pain threshold in rats; model of electro-acupuncture intervention mechanical pain threshold in rats to observe the same model group, but in the first 7 day one, with the HANS acupoint nerve stimulator 2Hz,1mA electro-acupuncture stimulate Weizhong and Huantiao for 30min, once a day for 7 days. Another observation is the inhibition effect of LTP electro acupuncture for the model group has been formed and maintained by, that is to say,observed the model group after the end of 3h given electro-acupuncture interventions, the parameter of time with the former, electro-acupuncture observed after 1h. Observation intervention effect of LTP of synaptic transmission use electro-acupuncture Huantiao and Weizhong to the SNI rats with mechanical pain threshold and spinal cord dorsal horn neurons.Results:1. After the operation of SNI, rats with bilateral mechanical pain threshold decreased significantly and more effect in operative side. At the same time, segment in sham group There were significant differences (P<0.01) while rats exhibited hind toe curling operative side, foot valgus, as well as pain and abnormal gait behavior changes.2. After use electro-acupuncture,the mechanical side surgery pain threshold by electro-acupuncture before 20.6±2.6g increased to 38.5±4.7g, significantly higher than the same time segment model group (P<0.01), while significantly reducing their pain and abnormal behavior.3. Model group C-fiber-evoked field potentials the rate of change in the HFS before induction, and after 3h induction, respectively for 69.9±30.8%,224.9±98.5% and 174.6±38.3%,, HFS induced C-fiber evoked field potential significantly higher than the rate of change of the basis of the average (P<0.01), while at the same segment in sham operation group at all time 3h after the induction of HFS was no statistical significance.4. Model of electro-acupuncture plus intervention in rats after electro-acupuncture, C-fiber-evoked field potentials the rate of change in the HFS before induction, and after 3h induction, respectively for 75.6±33.1%,73.3±20.3% and 62.9±20.8%, and the sham-operated group equivalent compared with the model group were significantly different (P<0.01).5. Group has been formed on the model of spinal cord dorsal horn neurons LTP, and to stabilize the maintenance of the SNI model rats 3 h the purposes of electro-acupuncture interventions, C-fiber-evoked field potentials from the electro-acupuncture before the rate of change of 182.8±29.7% down to 34.0±16.3%, with electro-acupuncture earlier significant differences (P<0.01).Conclusion:The production of neuropathic pain and spinal cord dorsal horn neurons in synaptic plasticity long-term potentiation, as well as the formation of the spinal cord dorsal horn neurons of the central sensitization has close relationship. Electro-acupuncture can significantly inhibit C fiber-evoked potentials of the formation of LTP, electricity for the maintenance of LTP has a significant role in blocking. At the same time, electro-acupuncture can significantly improve the rat model of neuropathic pain, pain behavior change. Electro-acupuncture may be prompted by inhibiting the spinal cord dorsal horn neurons in synaptic plasticity changes in the formation of reduced spinal cord dorsal horn neurons in sensitization exert analgesic effect.
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