Effect Of Bee Venom On Malignant Urinary Bladder Cells

Objective:Bee venom (BV) have biological activities of inhibition and apoptotic induction in human cancer cells. However, there is no report to address the molecular mechanism of V-induced intracellular Ca2+ release and apoptosis in human bladder cancer TSGH-8301 cells.The objective f this study is to investigae the effect of bee venom on malignant urinary bladder cells and pathways of apoptosis.Methods:We have used different experimental methods in morpholog,DNA damage to invetigate the apoptosis pathways induced by bee venom on malignant urinary bladder cell.Results:In this study, we have demostrated that BV has decreased the percentage of viable TSGH-8301 cells in flow cytometry. The result of flow cytometric analysis also found that BV promoted cell cycle G0/G1 arrest. And it increased the level of intracellular Ca2+ productions and lost the level of mitochondrial membrane potential(ΔΨm) in TSGH-8301 cells. The result of Immunostaining analysis,by confocal microscopy, showed that Ca2+ release, AIF and Endo G translocation into nucleus. In addition, BV-induced DNA degradation were confirmed by 4, 6-diamidino-2-phenylindole (DAPI) staining, Comet assay and DNA gel electrophoresis. Western blotting indicated that BV induced active forms of caspase-3,-8, and-9, and also promoted AIF and Endo G protein levels.Conclusion:Therefore, we suggested that BV inhibited the proliferation and induced apoptosis in TSGH-8301 cells through induction of intracellular Ca2+ and caspase-dependent pathway. Consequently, BV may be used for clinical treatment for urinary bladder cancer patient in future.