Modified XiChunyin And YuNvburusu On Experimental Rehabilitation Of Premature Ovarian Failure In Rats

Objective:To observe the rehabilitation of health effects by using the modified XiChunyin and YuNvburusu for premature ovarian failure in experimental rats, and discuss the mechanism of this mixed herb and food for premature ovarian failure to provide preventive health care and clinical treatment of new ideas.Methods:Healthy female SD rats are divided into three groups, blank-control group, herb food group, model group. Model group and herb food group are fed by 35% concentration of D-galactose to make model. The food of herb food group are added 15% concentration of the mixture of peanuts, red dates, soy, and the other herb:gouqizi, longyanrou, nvzhenzi, shudihuang, yinyanghuo, buguzhi, fupengzi, tusizi are extracted by ethanol, then gavage the rats with this liquide every day. These handles are all done 1 times/day,60 days successively. After 60 successive days,all rats of three group are fasting by 12 hours before blood sample taken in orbital vein on the 61th day,then separate the blood serum after centrifugalization and detect the content of FSH,E2 with radioinununity method. The rats are put to death by cervieal uertebra disloeated after the sample taken in the eye ball. Then put their ovaries on the electronic balance and calculate the ovarian index and retain them in formalin, gradient alcoholic dehydration, paraffin imbedding, mierotome section, routine HE dyeing and then oberve it under the light microscope.The results:(1) Weight of rats in each group before the experiment P>0.05, no significant difference. After the test, comparing the rats'weight of the model group with the blank-control group and the herb food group P<0.05, significant difference; the blank-control group is compared with the herb food P>0.05, no significant difference. (2) Comparing the model group to the blank-control group, FSH levels rise, E2 levels decreased, compared the two groups are significantly different P<0.01, P<0.01; comparing the herb food group to the model group, FSH levels decreased, E2 levels increased, the two groups were significantly different compared to P<0.01, P<0.01; the blank-control group's compared with the herb food group's, no significant difference P>0.05. (3) Comparing ovarian index of the herb food group's and the blank-controll group, P>0.05, no significant difference; the model group are compared with the blank-control group and the herb food group, its ovarian index was significantly lower, both P<0.05, significant difference. (4) The model group's tissue morphology of ovarian have obviously pathological changes, and the herb food group's were close to normal.Conclusion:The mixture of the herb and food in experimental can adjust the function of the hypothalamus-pituitary-ovarian, improve ovarian's function and play a estrogen-like role to improve weight loss, hyperhidrosis and other symptoms which can caused by ovarian failure, regulating the ovarian's response to gonadotropin, promoting development of follicle. The results of experiment can provide a theoretical basis for the development of health food for the female ovaries.