Experimental Research On The Amino Acids In Liver Disease With Physiological Fluid Method

Objective:(1) we use physiological fluid method to analyze the concentration of amino acids in patients of hepatic ciirrhosis, hepatocellular carcinoma and chronic hepatitis and found a standard model of physiological fluid analysis. (2) By comparing different serum amino acids concentration of hepatic ciirrhosis, hepatocellular carcinoma and chronic hepatitis,we investigate the model of amino acids metabolism of different liver disease. (3)By analyzing the amino acid changes of ascites in patients with cirrhosis,we evaluate their clinical significance.Methods:We randomly selected Tianjin Third Central Hospital of 60 patients as a medical control group.Experiment group was dedived into hepatic cirrhosis group(A group),hepatic carcinoma group(B group) and chronic severe hepatitis group(C group). In the first morning after admission,the blood samples were collected to carry out the liver function,blood coagulation and we adopted the physiological fluid method to analyze the amino acids. The ascites of 10 cases in liver cirrhosis patients with ascites was analyzed by physiological fluid method.Results:(1)The liver function:Compared with the N group, serum albumin(Alb),prealbumin of experimental group decreased gradually, and the serum aspartate aminotransferase, total bilirubin and total bile acid gradually increased, C group was the most significant compared with the N group (P＜0.05). (2)The blood coagulation:With the N group, the serum in prothrombin time, activated partial thromboplastin time and thrombin time of experimental group gradually extended, and C group increased most significantly(P＜0.05); the serum fibrinogen was decreased, and comparing with the N group, the difference was not statistically significant (P＞0.05). (3)The amino acids:Compared with the N group, the serum concentration of taurine(Tau),aspartic acid(Asp),isoleucine(Ile),arginine(Arg),cystathionine(Cysthi) and BCAA/AAA ratio of A group decreased significantly (P＜0.05); while the concentration of P-Alanine(β-Ala),β-Amino isobutyric acid(β-Aiba),γ-Amino-n-butyric acid(GABA),α-amino adipic acid(a-AAA),methionine(Met),tyrosine(Tyr) and phenylalanine(Phe) increased significantly (P＜0.05). We found that these amino acids(Asp,Cysthi,Ile,Arg,leucine(Leu), lysine(Lys))and BCAA/AAA ratio was decreased in B group(P＜0.05), and the concentration of Met,β-Ala,β-Aiba,GABA,α-AAA,Tyr,Phe,3-Methylhistidine(3Mehis) was higher than N group (P＜0.05). The concentration of Tau,Asp,Cysthi,ILe,Leu,Lys,Arg,glutamic acid(Glu),alanine(Ala),valine(Val) and BCAA/AAA ratio in C group was decreased(P＜0.05); while Met,β-Ala,β-Aiba,GABA,3Mehis,Tyr,Phe,threonine(Thr),1-Methylhistidine(1Mehis) increased significantly(P＜0.05). Between A and B group, the concentration of Val,Lys and Arg decreased significantly (P＜0.05), Tau and 3Mehis was increased significantly (P＜0.05). While compared A and C group, serum concentration of Thr,Met,Tyr,3Mehis was increased significantly (P＜0.05), Ala,Glu,α-AAA,Val,Leu and Arg was decreased significantly(P＜0.05). While compared B and C group, serum concentration of Thr,Met,Tyr was increased significantly (P＜0.05), the concentration of Asp,Glu,Ala,Val,Cysthi and a-AAA was decreased significantly(P＜0.05). (4)The amino acids concentration in ascites:With the N group, the concentration of Tau,Asp,Ser,Glu,Cysthi,Ile,Leu,α-amino-n-butyric acid(α-ABA) was decreased, while the concentrition of citrulline(Cit),cystine(Cys),Met,Tyr,Phe,β-Ala,β-Aiba,GABA and 3Mehis was increased significantly(P＜0.05).Conclusion:(1) We have a successful adoption of physiological fluid method in amino acid analysis, and by analyzing different amino acids in serum metabolites in various patients, we get more information about amino acids metabolism in petients. It provides an ideal method for the study of amino acid metabolism in liver disease. (2) By comparing amino acids concentration in serum of hepatic cirrhosis,hepatocellular carcinoma and chronic hepatitis patients with N group, we found they have different changes in amino acid metabolism and it provides some reference value for clinical diagnosis. (3) By comparing the difference between the serum amino acid concentrations in hepatic cirrhosis,hepatocellular carcinoma and chronic hepatitis, we found that a number of amino acids play an important role in the disease process, and establishing and improving metabolites resource database may be the direction in diagnosis and treatment of liver disease in the future. (4) Compared with the serum amino acids, the changesof amino acids in ascites and serum in hepatic ciirrhosis patients was consistent, it also reveals liver damage and provid the clues of metabolic abnormalities in hepatic cirrhosis.