Study On Mechanism Of Aromatic Yunpi Theorem And Its Representatives In D-IBS Rats

Objective:Irritable bowel syndrome (IBS) is a common disease with gastrointestinal disorder functions, the clinical syndromes include abdominal pain, diarrhea or constipation, and abnormal bowel evacuation habit. It is without changes of structure and biochemistry. The incidence of IBS is very high, but its pathological mechanism is not completely illuminated. At present, the visceral hypersensitivity characterized by changed dynamic and abnormal sense is one of the important pathogenesis. The disharmony of neuroendocrine-immunity network secretion is advanced cognition.The gastrointestinal peptides and cytokines play important roles in the mechanism as middle transmitters. The common treatment is limited to symptomatic, there is no method to root it out now, while Chinese medicine have satisfying curative effect. So there are broad foregrounds of application of traditional Chinese medicine.D-IBS belongs to the category of "diarrhea","abdominal pain" in traditional Chinese medicine. Its main mechanism is dysfunction of spleen and stomach, which leads to intestinal secretion of other features missing, liquid water can not infiltrate into the bladder,instead into colon, induce diarrea. Fragrant drugs can regulate the function of spleen and stomach, which has the features of elimination and benefit. Huoxiangzhegnqi is representative of fragrant drugs, which can regulate the activities of qi, eliminate the wetness-evil, invigorate the spleen and the stomach. It has good clinical effect on stopping diarrhea. But there is rare reports of research based on D-IBS. The purpose of our study is to explore the cure effect of Huoxiangzhengqi on D-IBS. By D-IBS rats model, we observe the opsonification of HXZQ on gut hormone and cytokine, in order to elucidate the adjusting visceral hypersensitivity and immunological function. We observe the effect of different ingredients in Agastache rugosa on some gut hormones, to discuss the diverse targets on D-IBS. All of our study will provide the scientific and reliable evidence for clinical application.Methods:1. D-IBS rats model is established by the combination of tied and intragastric administration Folium Cassiae.2. Groups with different concentrations of HXZQ were established. Through testing the changes of the general status such as the body weight, the diarrhea condition, tail flick assay, saccule extension in rectum and colon, we try to explain the effect of HXZQ in adjusting the visceral hypersensitivity preliminarily and determine the best dose.3. The level of SP,VIP,CGRP,ET and CORT were detected by radioimmunoassay and ELISA to show the adjusting effect of HXZQ on gut hormones.4. In order to explore the effect of different ingredients in Agastache rugosa, we detected the level of SP,VIP and ET.5. To discuss the adjusting effect of HXZQ on immunological function, we observed the atrophy degree of the spleen and thoracic gland, detected IL-1βand IL-2.Results:1. D-IBS rats showed obvious diarrhea, weight loss and immunological organs atrophied. There was no obvious injury by light microscope.2. HXZQ may obviously degrade the diarrhea rate (P<0.05), inhibit the weight loss (P<0.01),and improve the abnormal visceral hypersensitivity(P<0.05).The medium dose was the best.3. The medium dose of HXZQ can contribute to adjust the gut hormones, cut down the level of SP,VIP,ET, and keep the expression of CGRP(P>0.05).4. Agastache rugosa also can adjust gut hormones(P<0.05). And different indigents have different effect targets.5. The medium dose of HXZQ can improve the lower immunological conditions, degrade the expression of IL-1β(P<0.05) and elevate the level of IL-2 (P<0.01),except CORT(P>0.05).Conclusions:The model rats can induce the abnormal visceral hypersensitivity and immunological functions under stress. HXZQ, the representative of fragrant drugs, can adjust the expression of gut hormones and cytokines, improve the neuroendocrine-immunity network secretion to educe the therapeutical effect on D-IBS. Different indigents in Agastache rugosa have different effect targets, so in the procedure of making durgs we should not discard any indigent. Yunpi aromatic herbs and their representatives regulating stress-induced nerve-endocrine-immune network disorder, is one of the scientific content of regulating Qi in stomach and spleen, resuming their normal functions. Clinical treatment is different from the Liver pain Diarrhea, diarrhea accompanied by abdominal discomfort were its main syndromes.