Metallothionein 1A, 2A, 3, 4 MRNA Expressions In Peripheral Blood And Bucall Cell Of Patients With Non-small Cell Lung Cancer And Clinical Significance

Objective:To study Metallothionein(MT) 1A,2A,3,4-mRNA expressions in the peripheral blood and bucall cell of patients with non-small cell lung cancer and the clinical utility as molecular markers.Methods:30 cases with non-small cell lung cancer and 30 cases with benign lung tumor are selected as the research objects,including squamous cell cancer group, adenocarci-noma group,tuberculma group and inflammatory pseudotumor group.30 cases with non-small cell lung cancer are divided into two groups according to metastatic condition,including metastases group(17 cases) and no metastases group(13 cases).12 cases with lung laceration are selected as normal controls.MT1A,2A,3,4-mRNA expressions in the peripheral blood,bucall cell and lung tissue of all the cases are detected by RT-PCR.MT expressions in the lung tumor tissue and tumor surrounding tissue are deceted by immunohistochemistry. Comparative analyses of MT expressions at the mRNA and protein level are performed.Results:(1) MT-1A,2A,4 mRNA expressions in the peripheral blood of lung tumor group are down-regulated compared with those of normal control group.MT-1A,2A,4 mRNA expressions of squamous cell cancer group are significantly decreased compared with those of normal control group.MT-1A,2A,4 mRNA expressions of metastases group and MT-1A mRNA expression of no metastases group are down-regulated compared with those of normal control group.Compared with MT-1A,2A mRNA expressions of metastases group,those of no metastases goup are significantly decreased(P<0.05).Expressions change of the other groups else has no statistical significance.(2) MT-1A,2A,3,4 mRNA expressions in bucall cell of all the tumor group are up-regulated compared with those of normal control group.Each group has a significant change except for MT-4 mRNA of squamous cell cancer group compared with normal control group.MT-2A mRNA expression of tuberculoma group is significantly increased compared with that of squamous cell cancer group.There is no significant difference in other groups.(3) Normal group and tumor surrounding group as control groups are performed respectively in the lung tissue.â‘ MT-1A,2A mRNA expressions of squamous cell cancer's tumor group are significantly increased compared with those of normal control group(P<0.05),and MT-3 mRNA expression of adenocarcinoma's tumor group and MT-1A mRNA expression of inflammatory pseudotumor's tumor group are also significantly increased (P<0.05).â‘¡MT-2A mRNA expression of tumor group is significantly increased compared with that of tumor surrounding group in squamous cell cancer (P<0.05).MT-3 mRNA expression in tumor group of adenocarcinoma and MT-1A mRNA expression in tumor group of inflammatory pseudotumor are also significantly increased compared with those in tumor surrounding of their own (P<0.05).MT-3 mRNA expression of no metastases group had no significant change compared with that of normal contral group,while MT-3 mRNA expression of metastases group has a significant up-regulation (P<0.05).MT-3 mRNA expression of metastases group is significant increased compared with that of no metastases group(P<0.05).(4) MT expression of protein level in lung tissue is measured by immunohistochemical,and find that squamous cell cancer group and adenocarcinoma group are significantly increased compared with normal control group(P<0.01).Inflammatory pseudotumor group is also significantly increased compared with normal control group (P<0.05).squamous cell cancer group and adenocarcinoma group are significantly increased compared with their own tumor surrounding groups(adenocarcinoma group P<0.01,squamous cell cancer group and inflam matory pseudotumor group P<0.05).Compared with normal control group,metastases group and no metastases group are significantly inceased(metastases group P<0.01,no metastases group P<0.05).MT expression of metastases group is higher than that of no metastases group(P<0.05). Conclusions:MT-1 A mRNA in blood is expected to become an auxiliary diagnostic and metastatic marker of squamous cell lung cancer.MT-2A mRNA in bucall cell is expected to become an auxiliary diagnostic marker to distinguish squamous cell lung cancer from tuberculma.In lung tissue,up-regulation of MT-3 mRNA expression may be a sign of strong invasiveness.