Study Of The Anti-Diarrhea Effect Of Fuzhuan Tea

Diarrhea is a kind of stomach and intestines disease caused by many kinds of pathogenic factors with high incidence of a disease, which does a great harm to children's health. It is a worldwide public health problem. It is one kind of key prevented diarrhea disease in our country. According to the different pathophysiologi-cal mechanisms, diarrhea can be divided ihto the following several kinds:(1) There are many infiltrative solutes that can't be absorbed by intestines. (2) The water and electrolyte secrete excessively in intestines. (3) Inflammatory causes a great deal of liquide. (4) Intestines wriggles too fast. Diarrhea is usually caused by many kinds factors together.In China, Fuzhuan Tea is a kind of indispensable beverage for herd. Folk usually use Fuzhuan Tea to treat diarrhea. It is considered that the longer Fuzhuan Tea is stored, the more effective Fuzhuan Tea is. But the mechanism and anti-diarrhea function have not been reported before. The affection of Fuzhuan Tea on diarrhea animal models and mice intestine propulsion motility were researched in this paper. The restraining bacterium activity of Fuzhuan Tea on 6 kinds of bacterium was tested. That the function of anti-diarrhea and the difference between 3 kinds of Fuzhuan Tea produced in 1996,2002 and 2009 respectively was systemic researched. The results are as following.1 The function of restraining mice diarrhea induced by medicines of Fuzhuan Tea produced in different yearsWith berberine hydrochloride being a positive control and physiological brine being negative control, the function of anti mice diarrhea that caused by senna, castor oil and magnesium sulfate respectively of Fuzhuan Tea produced in 1996,2002 and 2009 by high dose group [5 g/(kg-bw)], middle dose group [1 g/(kg-bw)] and low dose group[0.2 g/(kg-bw)] was research. The different activities were tested between the three tea samples. In the model of senna diarrhea, the difference between the accumulated diarrhea times of the other groups apart from low dose group of Fuzhuan Tea produced in 2002, low and middle dose groups of Fuzhuan Tea produced in 2009 and that of physiological brine was significant (P<0.05) or very significant (P<0.01). It showed that Fuzhuan Tea was effective to anti-diarrhea caused by senna. The affection of high-dose of Fuzhuan Tea produced in 1996 was the same as that of berberine hydrochloride. In the model of magnesium sulfate diarrhea, it was active of Fuzhuan Tea produced in 1996, but that of Fuzhuan Tea produce in 2002 and 2009 was not obvious. The difference between the accumulated diarrhea times of high dose group of Fuzhuan Tea produced in 2009, all dose group of Fuzhuan Tea produced in 2002 and 1996 and that of berberine hydrochloride group was not significant. The longer the Fuzhuan Tea was store, the stronger function of anti-diarrhea it had. The activity of Fuzhuan Tea had positive correlations with the dose.2 The restraining of Fuzhuan Tea produced in different years on mice small intestine propulsion motility2.1 The effect of Fuzhuan Tea on normal mice alvine motilityThe function of advancing the motility of small intestine was significantly (P<0.05) of Fuzhuan Tea produced in 1996, Fuzhuan Tea high-dose group produced in 2002, middle dose group and high-dose group of Fuzhuan Tea produced in 2009. It was the activest of Fuzhuan Tea produced in 1996.2.2 The effect of Fuzhuan Tea on mice alvine propulsion motility caused by atropine sulfateAll the dose group of the three tea sample could inhibit normal mice alvine propulsion motility caused by atropine sulfatet movement (P<0.05 or P<0.01). The activity of Fuzhuan Tea had positive correlations with the dose. The activities of the high dose groups of the three tea samples were not statistically significant. Fuzhuan Tea produced in 1996 had the highest activity.2.3 The effect of Fuzhuan Tea on mice small intestine propulsion movement caused by neostigmine methylsulfateThe restraining function of all high-dosage groups of'Fuzhuan Tea were significantly (P<0.05). But that of low and middle dose groups was not significantly. The higher dose was, the better the function of Fuzhuan Tea. The function of Fuzhuan Tea produced in 1996 was the best. 3 The effect of Fuzhuan Tea produced in different years water extracts on bacteriumThe bacterium of Escherichia coli, Salmonella, Streptococcus hemolyticus, Staphylococcus aureus, Bacillus subtilis and Shigella were selected to test the anti-bacterium function of Fuzhuan Tea produced in 1996,2002 and 2009 respectively. The results showed that both of Fuzhuan Tea produced in 2009 and 2002 on Shigella respectively were the best. The diameter of anti-bacterium loop was 20.40 mm±0.87 mm and 18.10 mm±0.91 mm respectively. It was active of Fuzhuan Tea produced in 1996 only on Salmonella and Escherichia coli. But the activity was not obvious. According to the minimum restraining concentration, it was the most effective of Fuzhuan Tea produced in 2009, while it was the little effective of Fuzhuan Tea produced in 1996. The longer the Fuzhuan Tea was stored, the lower restraining activity the Fuzhuan Tea had.