The Expression Of ABCG2 And V-ATPase In Nsclc And Their Correlation With Drug-resistance

OBJECTIVE:To determine the expressions of ABCG2 and V-ATPase in NSCLC and the expression correlation between ABCG2 and V-ATPase. To investigate the correlation between their expression rates and drug-resistance.METHODS:Expressions of ABCG2 and V-ATPase were accessed with EnVinsion immunohistochemistry and RT-PCR in tumor samples. Their drug resistance to cyclophosphamide, 5-FU, GEM, TAX, DDP, Doxorubicin, NVB, HCPT, CBP were evaluated by MTT assay. The corresponding data was analyzed statistically.RESULT:Expressions of ABCG2 and V-ATPase were found both in the lung adenocarcinoma and squamous cell lung cancer. ABCG2 is expressed significantly differently among TNM stages of lung adenocarcinoma as well as among pathological grades of lung adenocarcinoma (P=0.028) and squamous cell lung cancer (P=0.000). The level of V-ATPase expression was associated with TNM stages of lung adenocarcinoma and pathological grades of squamous cell lung cancer. Additionally, significant correlation was found between expression of ABCG2 and V-ATPase in all samples, adenocarcinoma and squamous cell cancer (P<0.001). In the MTT assay, it shows higher resistance level in the adenocarcinoma than in the squamous cell lung cancer. The correlation between the expression of ABCG2 and V-ATPase and the drug resistance level to cyclophosphamide, CBP and DDP is significant .CONCLUSION:The level of ABCG2 expression is associated with TNM stages of lung adenocarcinoma as well as the degree of the malignancy of both squamous cell lung cancer and lung adenocancinoma. The levels of V-ATPase expression were associated with TNM stages of lung adenocarcinoma and the degree of malignancy of squamous cell lung cancer. Significant correlation was found between expression of ABCG2 and V-ATPase, which indicated that they may co-work to participate the machanism of anticancer drug resistance.