| Objective1.To observe the effects of herbal medications—Fuyuan capsule on articular cartilage histomorphology,expression of IL-6 and bFGF in experimental knee osteoarthritis in rats.2. To compare the preventive and therapeutic effects of Fuyuan capsule and tetracycline in experimental 0ste0arthritis in rats.3.To analyze the correlativity between Mankin'S score and the level of IL-6 and bFGF in articular cartilage,and to investgate the mechanism of Fuyuan capsual on the prevention and treatment of OA.Methods1. Modeling and grouping Sixty health SD male rats were randomly devided in to six groups: normal control group,model group, tetracycline group ,high Fuyuan capsule group, middle Fuyuan capsule group, and low Fuyuan capsule group.Except control group,all were established OA modle with Hulth technique.One week later,rats in tetracycline group and Fuyuan capsule groups were given tetracycline and Fuyuan capsule by pouring stomach for ten weeks; at the same time,control group and modle group were given the same amount of NS.2.Macropathology and micropathology Articular cartilage were obtained ten weeks later. pathological change of articular capsule,membrana synovialis,arthrodial cartilage and presence of joint effusion were evaluated through macroscopic observation; the structure of cartilage,quantity of chondrocyte and integrity of tidal line were observed through Microscopic observation by light microscope.Histomorphology of cartilage were evaluated according to macroscopic grading score and Mankin's score.3.Expression of IL-6 and bFGF in articular cartilage Detected by immunochistochemistry technology. Pearson's Correlation analysis was done to determine the grade point index number and the level of IL-6 and bFGF in articular cartilage.Results1. Morphology Changes of cartilage in each group The macroscopic grading score and Mankin'S score in model group,Fuyuan capsual groups and tetracycline group were higher than that in contral group,the model group is the highest one; As the dosage of Fuyuan capsule increasing,the Mankin's score in Fuyuan capsule groups decreased;the Mankin'S score in middle and low Fuyuan capsule group higher than that in high Fuyuan capsule group and tetracycline group; the Mankin's score between tetracycline group and high Fuyuan capsule group have no significancant difference.2. Expression of IL-6 and bFGF in each group2.1 IL-6: Expression of IL-6 in model group,Fuyuan capsual groups and tetracycline group were higher than that in contral group,the model group is the highest one; expression of IL-6 in three Fuyuan capsual groups have significancant difference, the high Fuyuan capsule group is the lowest one(P<0.05); expression of IL-6 between tetracycline group and high Fuyuan capsule group have no significancant difference. As the pathogenetic condition progressing, expression of IL-6 become higher and higher.2.2 bFGF: Expression of bFGF in contral group distributed uniformly,lower than that in model group(P<0.01); expression of bFGF in Fuyuan capsual groups and tetracycline group are higher than that in model group(P<0.01); in three Fuyuan capsual groups, expression of bFGF have significancant difference, the high Fuyuan capsule group is the highest one(P<0.05); expression of bFGF in high Fuyuan capsule group is higher than that in tetracycline group(P<0.05).3.Relationship between IL-6 and bFGF expression and Pathological changes of cartilage in OAPearson's correlation analysis showed that the level of IL-6 in the articular cartilage was positively correlated with the subindex of arthrosis pathological change and the correlation coefficient was 0.9638(P﹤0.01); no significant correlation was found between the level of bFGF in the articular cartilage and the subindex of arthrosis pathological change, the correlation coefficient was 0.1540(p>0.05).Conclusion1. Fuyuan capsule can mitigate histological changes of cartilages , down-regulate the expression of IL-6, up-regulate the expression of bFGF, showing a dose-dependent effect within a certain range.The treatment effect of high-dose Fuyuan capsule is identical to tetracycline in experimental rats OA . Fuyuan capsule may be by acting on the cytokine network, inhibitng chondrocyte apoptosis and matrix degradation and promotlng proliferation show an intervention on rats OA.2. Expression of IL-6 in articular cartilage showed positive correlation with histomorphologic subindex of Mankin's score,no significant correlation was found between the level of bFGF in the articular cartilage and the subindex of arthrosis pathological change.
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