Related Research Of Cytokines In The Pathogenesis And In The Treatment Of Osteoarthritis

Objective The purpose of this study is to determine interleukin (IL)-17 concentrations in serum and synovial fluid from patients with knee osteoarthritis, and evaluate their correlation with disease severity. This study was carried out to observe the effect of basic fibroblast growth factor(bFGF)on rat bone marrow mesenehymal stem cells(BMSCs)and its potentials of differentiation in vitro.Methods (1)Serum and synovial fluid were collected from patients with primary knee osteoarthritis;age-and sex-matched healthy control subjects provided serum samples. This study was conducted retrospectively. IL-17 was quantified by enzyme-linked immunosorbent assay. Osteoarthritis severity and grade were assessed using the Lequesne index and Kellgren and Lawrence (KL) grading system, respectively.(2) Rat BMSCs were cultured with a medium treated with bFGF at concentrations of 0,1,10,100ug/L. After 72 h of treatment, the effect of bFGF on BMSCs viability was tested using methyl thiazol tetrazolium (MTT) assay for detection of optical density. The expressions of CD44 of BMSCs were investigated by immunocytochemical analysis and reverse transcription-polymerase chain reaction(RT-PCR) assay.Results (1)Serum IL-17 concentrations were significantly higher in patients (n=98) than in controls(n=50). In the patient group, the synovial fluid IL-17 concentration increased significantly with KL grade and was significantly positively correlated with Lequesne index (r=0.6232).(2) After treatment of bFGF at concentration of lug/L. the optical density(0.334±0.036)in 1 ug/L bFGF group was markedly increased compared with control(0.251±0.033)(P<0.05). The integrated optical density of CD44 in 1 ug/L bFGF group was significantly increased, compared with control(P<0.01). The mRNA expression of CD44 in lug/L bFGF group was significantly increased, compared with control(P?0.01).Conclusions (1)The synovial fluid IL-17 concentration could represent a useful biochemical marker to reflect knee osteoarthritis severity and progression. After treatment of bFGF at concentration of 1ug/L, bFGF can induce the proliferation of BMSCs and preserve its potential of differentiation.