Upregulation Of Albumin D-site-binding Protein In The Brain Of Patients With Intractable Epilepsy

Objective The mechanisms that underlie the pathogenesis of intractable epilepsy (IE) are still unclear. This study aimed to investigate the expression of albumin D-site-binding protein (DBP) and mitogen-activated protein kinases (MAPK) in the temporal lobes of patients with IE, in order to explore the possible roles of DBP in the pathogenesis of IE.Methods Expression of DBP and MAPK was detected with immunohistochemistry and double-label immunofluorescence staining with DBP/MAPK in 35 patients with IE, and the data were compared with those of 15 controls.Results DBP expression in IE (0.4737±0.0262) was significantly higher compared with controls (0.2476±0.0229, P﹤0.05), and MAPK expression in IE (0.2935±0.0106) was likewise higher compared with controls (0.2370±0.0125, P﹤0.05). DBP and MAPK were mainly expressed in the cytoplasm of neurons, and the double-label immunofluorescence staining showed that DBP and MAPK occurred in the same neurons.Conclusions The expression of DBP and MAPK in epilepsy patients was up-regulated, suggesting a possible pathogenetic role in IE.