Study On Protective Effects Of Sepia Ink Polysaccharides On Chemotherapy

Objective: Cyclophosphamide (CP) is an alkylating agent, the most commonly used anti-tumour drugs in practice, but it also has intense side effects on normal tissues when it kills the tumour cells, which lead to the limitation of CP to some extent. Sepia ink polysaccharides (SIPS) is a kind of active substance extracted from sepia ink, the research on sepia ink and on peptidoglycan or melanin from sepia ink has been done by the former researchers, but the paper on the activity of SIPS is seldom seen. So we created chemotherapeutic injury model by injecting with CP and administered SIPS orally on rats or mice, examined the changes of bio-chemistry parameters in some organs, blood indexes as well as histopathological changes, further explored the restorative effects of SIPS on injuries of ovary, spleen, liver, lung, kidney, heart induced by CP and it's possible mechanisms. Our results provide the scientific evidence of reasonable exploitation and application of sepia ink.Method: Healthy Kunming mice were divided into 8 groups, male and female mice share equal quantity. Various concentrations of SIPS were administered orally to the CP intoxicated animals to optimize the maximum efficacy of SIPS in the minimum dose, determined by the changes of peripheral blood profile, Glutamic-pyruvic transaminase, DNA contents in bone marrow, and uric acid in serum, and repeated it.40 female S.D. rats were randomly divided into 4 groups, consisting of 10 rats each: control group, model group, SIPS group and treated group, examined the changes of bodyweight, index of ovary, hormone level in serum, antioxidative capacity in blood and in ovary.40 S.D. rats were randomly divided into 4 groups, male and female rats share equal quantity in each. The way of grouping was the same with above, determined the changes of peripheral blood profile, index of spleen, contents of DNA in bone marrow, antioxidative capacity in spleen and histopathological changes after the experiment period.40 BALB/C mice were randomly divided into 4 groups, male and female mice share equal quantity in each. And also the way of grouping was the same with above, the changes of index of liver, kidney, lung, heart and antioxidative capacity in them, ALT and AST in liver, and total bilirubin (TBIL), albumin,blood urea nitrogen (BUN), creatinine,β-N-Acetyl-glucosaminidase (NAG )in blood were examined. Results: The yield of SIPS that extracted by us was about 1%, the contents of protein is also 3.07% after being gotten rid of protein by Sevag method, the sulfate content is 6.297%. After twice tests, we confirmed that the optimal concentration of SIPS was 180mg/kg among various concentrations tested by us.Compared with control group, CP can change the contents of P, E2, FSH in blood and the T-AOC in ovary tissue significantly (P<0.01 or P<0.05), but had no obvious effects on T-AOC in serum (P>0.05), and it also had different effects on different kinds of enzymes in blood or organ (P>0.05 or P<0.05) .CP can sharply reduce the quantity of follicles in different phases, and there was not mature follicles in ovary which had focal fibrosis in histiocyte. compared with model group, SIPS can ameliorate incoordinately the changes of parameters induced by CP (P<0.05 or P>0.05).CP can affect significantly the contents of erythrocytes, leukocytes, hemoglobin, platelets in blood , DNA in bone marrow(P<0.01), organ index of spleen(P<0.05), as well as the contents of MDA and the activity of SOD in spleen(P>0.05). SIPS had no obvious effects on the above parameters compared with control group, but can incoordinately change the parameters induced by CP(P<0.05 or P<0.01)except for contents of erythrocytes and hemoglobin and organ index of spleen(P>0.05). SIPS can inhibit obviously the changes of index of liver, the activity of ALT and AST in liver , contents of BUN in blood induced by CP (P<0.01) , but had no effects on albumin, NAG, creatinine, uric acid level in blood. Meanwhile, SIPS also can inhibit incoordinately antioxidative capacity changes in above organs induced by CP.Conclusions: SIPS can protect female reproductive system, hemopoietic system and some organs from chemotherapeutic injuries induced by CP.