Experimental Study Of Daphnetin On Its Anti-tumor And Toxicology Effects

Objective: To evaluate the toxicity of daphnetin, to study its antitumor activity furtherly, and to provide the theory foundation for the development of daphnetin as medicine.Method: 1. Developing the MTT assay in the vitro of daphnetin, and studying the effect of daphnetin with different concentration to the SMMC-7721 and S180 cells when were proliferating. 2.Evaluating the toxicity of daphnetin by acute toxicity tests, Ames tests, and micronucleus test.Results: 1. When the daphnetin worked on SMMC-772 cells with the concentration of (μg/ml) 1.95,3.90,7.81,15.62,31.25,62.5,125,250, the daphnetin inhibitory rates were51.48±1.65,60.32±2.49,68.97±2.51,74.23±2.56,78.51±0.61,79.35±1.22,83.6±1.47,87.1±2.35; when the daphnetin worked on S180 cells, the daphnetin inhibitory rates were7.52±3.70,14.96±4.90,38.17±12.22,40.64±12.52,52.01±2.67,69.10±4.31,76.28±10.62,86.54±4.24 respectively.2. Mice were treated with the daphnetin with 100/kg.bw over 175 times to human'dose which were lasting 7 days without detecting any toxic signs and change of weights.3. The reverse mutation of daphnetin with different concentration were less than twice times to the control group without dose-dependent action and the Ames array were negative.4. There was no significance between the daphnetin groups and the control groups(p>0.05), thus significance observed between the CY group and negative group(p<0.05), which indicated that daphnetin had no inhibitory and inherent toxicity activity on morrow cells.Conclusion: 1. The daphnetin had inhibitory activity to SMMC-7721和S180 cells in some range with dose-dependent effect. 2. The daphnetin had no significantly toxicity activity without mutagenesis.