Expression Of E2F-1, NF-κB/P65 And P-gp In Diffuse Large B Cell Lymphoma And Their Clinical Significance

Objective:To investigate the expression of E2F-1,NF-κB/ P65 and P-gp in diffuse large B cell lymphoma and their relationship with clinicopathologic parameters and prognosis.To provide a theoretical basis for the judgement of the prognosis and treatment.Methods:The expression of E2F-1, NF-κB/ P65 and P-gp in 61 cases of diffuse large B cell lymphoma tissue and 15 cases of reactive hyperplasia lymph nodes were detected by using immunohistochemical method.Their correlation and their relationship with clinicopathologic parameters and prognosis were analyzed by using SPSS 12.0 software.Results:(1) Among the 61 cases of diffuse large B cell lymphoma tissue,the positive rates of E2F-1 was 42.6%(26/61),which was obviously lower than reative hyperplasia lymph nodes(P<0.05);the positive rates of NF-κB/ P65 and P-gp were 52.5% (32/61)and 54.1%(33/61),respectively.The rates were significantly higer than reative hyperplasia lymph nodes(P<0.05).(2) The expression of E2F-1 was negative correlation with the expression of NF-κB/ P65,and negative correlation with the expression of P-gp(r=-0.404, P<0.05). The expression of NF-κB/ P65 was positive correlation with expression of P-gp(r=0.638, P<0.05). (3)The expression of E2F-1 had no significant relationship wih gender,age,Ann Arbor stage,IPI score,LDH and B sympton(P>0.05); The expression of NF-κB/ P65 significantly correlated with Ann Arbor stage and IPI score(P<0.05),but not with the gender,age,LDH and B sympton(P>0.05); The expression of P-gp had correlation with IPI score(P<0.05),but had no correlation with gender,age, Ann Arbor stag ,LDH and B symptom (P>0.05). (4)The 5-year survival rate of the E2F-1 positive patients was significantly higher than the E2F-1 negative patients,the difference between them was significant(P=0.0301); The 5-year survival rate of the NF-κB/ P65 positive patients was significantly lower than the NF-κB/ P65 negative patients, the difference between them was significant(P=0.0116); The 5-year survival rate of the P-gp positive patients was lower than the P-gp negative patients, but the difference between them had no significance(P=0.1232).Conclusions:(1) In diffuse large B cell lymphoma,the expression of E2F-1 is significantly reduced;the expression of NF-κB/ P65 and P-gp is significantly increased.(2) In diffuse large B cell lymphoma, the expression of E2F-1 is negative correlation with the expression of NF-κB/ P65,and negative correlation with the expression of P-gp;the expression of NF-κB/ P65 is positive correlation with expression of P-gp.These suggest that they may have a certain degree of interaction in diffuse large B cell lymphoma.(3) The positive expression of NF-κB/ P65 is positive correlation with Ann Arbor stage and IPI score,which suggest that the positive expression of NF-κB/ P65 may promote the development of diffuse large B cell lymphoma.(4) In diffuse large B cell lymphoma,the high expression of P-gp suggests that there are primer multidrug resistances.(5) The negative expression of E2F-1 and the positive expression of NF-κB/ P65 are unfavourable prognostic indicators in diffuse large B cell lymphoma.