Effect Of Qu Yi Kang On MMP-2 And MMP-7 Of Endometriosis In Rats

Objective:By means of the Endometriosis(EMS) rats model,The observation Qu Yi Kang to EMS rats model endometriotic(EOS) the matrix metal protein-2,-7 expression influence,Further discussion the nosogenesis and treatment endometriosis of traditional Chinese medicine Qu Yi Kang.Methods:(1)60 healthy female SD rats were randomized into 6 group(A group:Blank group;B group:Model group;C group:Qu Yi Kang small dose group;D group:Qu Yi Kang middle dose group ;E group:Qu Yi Kang big dose group; F group:Danazol group. Besides A group is control group,other 5 group of even good surgery autotransplantation law establishes EMS rats model. After making the membrane success,A,B groups were fed with 0.9% Nacl (2ml/100g);C,D,E groups were fed with Qu Yi Kang fliud, separately including crude drugs 1g/mL,2g/mL,4g/mL ;F group was fed with Danazol fliud, including crude drugs 0.016g/(mL·100g).each time 2ml.After four weeks,all rats were skilled at the metaestrs stages.(2)After before the observation makes the membrane,makes the membrane latter 5 week rats body weight the change and the treatment, each group of dystopia internal membrane volume change.(3) Under the light microscope observes each group Endometrium(EM) and EOS pathology change.(4) The immunity histochemical method analyzes in EM,EOS MMP-2,7 expression changes,discusses Qu Yi Kang to treat EMS the possible mechanism.Results: (1)Makes the membrane,makes the membrane latter 5 week rats body weight with the normal group comparison not obvious difference (P>0.05).(2)Applies drugs 4 weeks later,Danazol group and Qu Yi Kang groups dystopia EOS volume to reduce, has the obvious difference with the model group comparison (P<0.05). Qu Yi Kang groups and Danazol group dystopia EOS volume comparison not obvious difference (P>0.05). (3)Under the light microscope observes discovery model groups EOS to grow exuberantly, Danazol group,Qu Yi Kang groups to be able to cause the EOS gland epithelial cell atrophy obviously.(4)Makes membrane each group of dystopia internal membrane MMP-2,7 expression normal group high,the difference has statistics significance (P<0.05). Applies drugs 4 weeks later,Qu Yi Kang big and middle dose groups,Danazol group MMP-2,7 expression that to compare obvious difference with the model group (P<0.05), that to compare not obvious difference with Danazol group (P>0.05).Conclusion:(1)By Using its won Intima Planting Method success to establish the EMS rats model, but needed is grasping the anaesthesia dosage precisely,the standard followed the aseptic technique under the premise to carry on, will bleed, the infection control in the lowest degree,enhanced makes the membrane survival rate.(2) MMP-2,7 is playing the great importance in the EMS occurrence development the role, the two expression level's markup is one of internal membrane planter key links.(3) The Qu Yi Kang various dosage to be possible to suppress in the EMS rats model dystopia internal membrane the MMP-2,7 expression level, Qu Yi Kang is big, dosage effect for good, through suppresses EMS rats dystopia internal membrane MMP-2,7the excessive expression and the enzyme activity, further reduces its attack, the shift, planter ability, is helpful in the control dystopia infection's unceasing growth, this possibly is Qu Yi Kang to treat one of EMS sickness mechanisms. (4) The Qu Yi Kang various dosage not to have the untoward effect to the EMS rats model.