Novel Metal Matrix And Application In In-vitro Diagnosis Of Auto-immune Diseases

Metabonomic analysis of biological samples is significant for clinical diagnosis and treatment.Matrix-assisted laser desorption ionization mass spectrometry(MALDI)has become a commonly used analytical method in metabolomics due to its high resolution,high sensitivity and fast detection speed.However,conventional organic matrices significantly hinder the analysis of small metabolites owing to the high background noises in the low mass region(m/z<500),and "sweet-spot effect".Novel metal materials with high signal-to-noise ratio and no background signals are promising alternative to address the above challenges.In this work,we reported the synthesis of novel metal materials and applied them to detect small metabolites in biological samples(e.g.serum and urine).Meanwhile,we obtained the serum metabolic profiles of autoimmune diseases by MALDI,and achieved the in-vitro diagnosis of autoimmune diseases.The uniform surface morphology,stable nanostructure and effective ultraviolet absorption of the prepared materials greatly improve the efficiency of desorption and ionization.Our method based on this material offers high sensitivity(~nmol),low sample consumption(~nL)and ease of sample pretreatment.The novel metal matrix exhibited sensitive detection of small metabolites in biological samples.In addition,we constructed a metablome database for anto-immune diseases,and distinguished the patients with healthy controls with a sensitivity up to 90.68%,a specificity up to 89.67% and AUC up to 0.9627.We also identified nine metabolic pathways that are highly associated with the disease,which would guide the further biological research in the future.Our work would contribute to the design of high-performance metabolic analysis platform,the accurate diagnosis of autoimmune diseases and the research of disease prediction.