Infection And Epidemiology Analysis Of Bartonella Spp. In Special Population And Animals

Objective:To investigate the infection condition and correlating epidemiology information of Bartonella spp. in special population (professional animal contactors and people bitten by dogs or cats) and animals (pets, vagrants and laboratory animals), and to get Bartonella isolates of different hosts. Make clear the Bartonella infection and explore potential risk factors.Methods:EDTA-anticoagulated blood samples, serum and correlated epidemiology information were collected from 193 people and 478 animals in Shandong province and Beijing. All isolates were grown on brain heart infusion agar plates containing 5% defibrinated sheep blood. The plates were incubated at 37℃at a humidified with 5% CO2 environment for 4 weeks or longer. All Bartonella-like isolates were examined by gltA and tRNA PCR. Sequencing citrate synthaes(gltA) gene,16S,16S-23S rRNA ITS,ftsZ,ribC were carried out and homology similarities were calculated using DNASTAR5 software package. The phylogenetic trees were inferred from each bootsrap sample, using the neighbor-joining methods as executed in the MEGA4 software. Use the indirect immunofluoescence test to detect Anti-Bartonella hensellae IIFT(IgG) in pelple and cats, and anti-Bareonella quintana IIFT(IgG) in Primates. Extract DNA from EDTA-anticoagulated blood and use polymerase chain reaction(PCR) to amplify citrate synthaes(gltA) gene and tRNA gene, and analysis the homology. Use SPSS13.0 to analysis the epidemiology information and the results of laboratory.Results:1, The positive incidence of anti-Bartonella henselae IIFT (IgG) in investigated people is 33.7%, and there in no statistical significance between people bitten by dogs or cats, fox and marten feeders and lab animal feeders. Bitted pelple in city has a higher positive incidence than in rural area, and people bitted by cats has a higher positive incidence than these bitted by dogs. Other variables have no statistical significance.2, We get 22 Bartonella henselae isolates from cats. The vagrant cats have higher bacteremia incidence (30.4%) than domestic cats(4.8%), but the antibody positive incidence has no statistical significance. Cat with fleas, young kitten have higher bacteremia incidence, especially kitten under 1 year old.3, The sensitivity and specificity are not satisfactory when using indirect immunofluoescence test (IFA) to detect cats bacteremia. The positive predictive value is 94.8%, so we have much confidence to determine a cat with negative I FA has no bacteremia.4, We get a B. henselae isolate from dog and 2 B. quintana isolates from Macaca mulatta. This is the first time to find B. henselae infection in dog in China, and the first time to find B. quintana in Macaca mulatta. It suggests Bartonella epidemiology is more complex than expected and ask we do more work about host specificity.