| bjective:Research has good absorbency, including high content, better stability of its preparation, capsule extraction and purification process, preparation technology, quality standard,the antioxidant activity test, fingerprint technology research and experiment study of the shaping.Methods:1.The extraction and purification process research:to Erigeron Canadensis L. in amount of extracting effective components rutin as index,using ultraviolet spectrophotometry to the content of rutin in material,concentration, washed with for the influence factors, velocity of L9 (34)orthogonal test Erigron Canadensis L. purification extraction process optimization refined products I,With Erigeron Canadensis L. rutin in and for quercetin index,extracted by HPLC, materials, content, size ratio for the factors affecting the eluate velocity L9 (34)orthogonal experiment refined products II extraction process optimization.2.Experiments on antioxidant activity of Anti-cancer effective components and its refined products was determined by the DPPH scavenging activities to the optimum extraction.3.API standard of quality research:TLC method for APIs,scutellarin and rutin and quercetin, and luteolin effective ingredients qualitative identification, Determined by HPLC method for content, chromatographic column:C18 column (4.6mmx250mm,5μm) (Agligent 1100), Mobile phase:methanol a 0.5% glacial acetic acid and water; 360nm velocity detected wavelength,column temperature:30 degrees.4.Fingerprint research:by high performance liquid chromatography (HPLC) based on fingerprint, establish including common peak, similarity and peak area ratio of Erigeron Canadensis L. API fingerprint quality control standards,and 10 batch samples tested.5.In capsule molding process in hygroscopic and liquidity index such as the type of material,respectively, dosage and wetting agents,etc, and determines the preparation of preparation and molding process is established quality standards, but also the moisture content time limit the difference of the project.Results:1.the Erigeron Canadensis L. refined products optimum extraction process:Erigeron Canadensis L. refined products I optimum technological conditions for A2B3C3.In the concentration of ethanol with agent for 70%,material proportion is 3g add more than 100 mL, adsorption velocity for 1.5 mL/min.Get effective parts of effective constituents anti-cancer high content by Erigeron Canadensis L. refined productsⅡ.Erigeron Canadensis L. anti-cancer effective composition content and content of quercetin index, A2B1C2 formulated as the best extraction solution, namely material than 0.50 for 150:for thus, diameter ratio and velocity of 1.0 mL/min.2.The antioxidant experiments showed that IC50 respectively antiradical efficiency was 3.58,6.17,and 8.00 when the flavonoids content reached at 28.7%,53.2%,and 77.5%,respectively.3.Erigeron Canadensis L. antitumor Capsule drug quality standard research.:By thin layer chromatography in luteolin for APIs, quercetin and scutellarin, by uv spectrophotometry and HPLC method for effective drug were,The moisture of APIs,heavy metal,such as disintegrating tested,accurate and reliable quality standards.4. fingerprint research:Erigeron Canadensis L. API preliminary comparison fingerprint,marked by HPLC Erigeron Canadensis L. four of the materials were peak.5.Optimization of Erigeron Canadensis L. antitumor capsule formability process and quality standards:with preparation for 20% of starch and accessories for 80% ethanol wetting agents,choose to use 1 capsule shells capsule, craft relevant quality standards, good stability. Conclusion:Making good absorbency, including high content, better stability of its preparation, capsule extraction and purification process,preparation technology, quality standard,the antioxidant activity test, fingerprint and preparation for the experiment formability, Erigeron Canadensis L.antitumor capsule further development has laid a good foundation.
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