Research On Metabonomics With Syndrome Type Of Hypertension And The Medical Intervention

Objective: Observed the metabonomics features on hyperactivity of liver fire syndrome and dampness and phlegm syndrome of essential hypertension. Metabolites were compared between two syndrome for seeking the potential biomarkers. Meanwhile,observed the changes in metabolomics after treatment of hypertension and the effect of antihypertensive drugs on the metabolism of spectrum.Methods:This study selects patients with essential hypertension as subject, among which 10 patients with hyperactivity of liver fire syndrome as Group a,10 cases of dampness and phlegm syndrome as Group B and 15 health comparison cases as Group K. The selection is made under the Case Selection Criteria. The participants are chosen from Department of Cardiology in Jiangsu Provincial Hospital of TCM and the physical examination center. The vital sign and biochemical parameters of each groups of participants are observed; 12 hours of fasting venous blood was collected; 10 of 20 patients among Group a & b are taken antihypertensive medical for 4 weeks before obtain 24 hours of fasting venous blood. Thus these 10 patients are arranged in a new group (Group y). After the proper treatment of samples, a wide spectrum of plasma metabonomics analysis is organized on the blood sample of patients with two syndromes, healthy people and people in treatment. Using basic statistical methods and metabolomics approach to analyze the results of the blood sample.According to the principal component score plot of samples, can distinguish differences between groups and related biological markers can be obtained.Results:(1) Dampness and phlegm syndrome group of patients have significant difference between the control group in BMI, TG, HDL-C, LDL-C (P<0.05), there were statistically significant.(2) Hyperactivity of liver fire syndrome,dampness and phlegm syndrome and healthy population control group have differences in metabolic spectra.(3) There are certain differences in metabolism spectrum between hyperactivity of liver fire syndrome and dampness and phlegm syndrome. The two syndromes have significant difference in Phenylalanine, glutamic acid (P<0.05).(4) After treatment, patients have significant changes in metabolic spectrum. There are significant differences in the compounds such as:fructose, phenylalanine, 9,12-octadecadienoic acid (P<0.05)Conclusions:(1) The use of metabonomics methods can successfully distinguish between hyperactivity of liver fire syndrome, dampness and phlegm syndrome and control group.(2) Preliminary view that phenylalanine, glutamic acid can be used as identification of hyperactivity of liver fire syndrome and dampness and phlegm syndrome to biological markers of metabonomics. (3) Preliminary view that fructose, phenylalanine,9,12-octadecadienoic acid has significant change after using antihypertensive drugs. (4) Considered overweight, obesity and dyslipidemia affect the incidence of hypertension dampness and phlegm syndrome.