| ObjectiveTo investigate the telomerase activity in human granulosa cells of poor responder patients and to evaluate its role in the mechanism involved in poor ovarian responder.Methods45 samples of human granulosa cells were obtained from patients undergoing IVF-ET treatment. The telomerase in human granulosa cells was determined by Real-time PCR and the telomerase activity was compared between the poor responder group(experimental group,n=19) and the normal responder group (control group,n=26).Results(1)The E2 level on the day of injecting HCG(1080±861.22 vs. 3543±1772.41 pg/ml),the number of retrieved oocytes(4.37±1.16 vs. 11.77±1.97)and good-embryos(2.07±1.60 vs.5.65±4.20)in POR patients were significantly lower than that in NOR patients(P<0.05). But there was no significantly difference in the fertilized rate(71.9% vs. 73.4%),cleavage rate(93.0% vs.92.5%),good-embryo rate(59.4% vs.56.5%),implantation rate(14.6% vs.24.0%)and clinical pregnancy rate(31.6% vs.42.3%)between the two groups(P>0.05). (2)Telomerase activity could be detected in all human granulosa cells samples from patients undergoing IVF-ET treatment.(3)The telomerase activity in ovarian granulosa cells decreased with age and serum basal FSH.(4)The telomerase activity was significantly lower in poor ovarian responder group than that in normal ovarian responder group(P<0.05).Conclusion(1)In young poor ovarian responders, the number of retrieved oocytes was significantly lower than that in normal ovarian responders. But there was no significantly difference in fertilization rate, good-embryo rate,implantation rate and clinical pregnancy rate between the two groups.(2)The telomerase activity in ovarian granulosa cells decreased with age and serum basal FSH,suggests that telomerase may play an important role in evaluate the ovarian function.(3)The lower activity of telomerase in granulosa cells may related to the mechanism of poor ovarian response. |
