The Research Of Tumor-derived EGFL7 Effect On Human Umbilical Vein Endothelial Cell Biology Behavior

Party I The study on clinical significance of the expression of EGFL7 in gastric carcinomaObjective:To explore the expression of EGFL7in gastric carcinoma and its correlation with tumor clinical and pathological features.Methods:The expression of EGFL7 protein and MVD in 45 cases gastric carcinoma were detected by immunohistochemical methods.Result:The expression of EGFL7in the team which has lymph node metastasis (84.6%) is higher than the team which hasn't (57.9%),in the serosal invasion team (84.4%) is higher than the team which doesn't have serosal invasion (46.2%). The expression of EGFL7 in gastric carcinoma is related with tumor invasion and lymph node metastasis (p<0.05),but isn't related with the patients'age, sex and tumor differentiation (p>0.05)The MVD of poorly differentiation team (37.62±10.42)is higher than the well-differentiation team (27.91±9.93) and differentiated team (28.4±9.18). The serosal invasion team's MVD(34.25±10.43)is higher than the team which is not (26.69±8.66),the team's MVD which has lymph node metastasis (33.00±9.99) is higher than the team which hasn't (25.94±9.34).The MVD is related with tumor differentiation, invasion and lymph node metastasis, but isn't related with the age and sex (p>0.05)MVD in the tumor which express EGFL7 is 33.80±10.56, that is higher than the MVD in the tumor which don't express EGFL7 (26.00±7.21) (p<0.05).Pearson analysis shows MVD and EGFL7 expression is positive correlation (r=0.313,p<0.05)Conclusion:High expression of EGFL7 would progress the infiltration and metastasis of gastric caicinoma. The mechanism maybe related with angiogenesis. PartyⅡStudy on the effect on human umbilical vein endothelial cell biology behavior when EGFL7 gene silence in gastric carcinoma cellObjective:To explore the effect on HUVEC's biological behavior when silence EGFL7 in gastric carcinoma cell.Methods:Use immunocytochemistry and western blot to defect EGFL7 expression in 4 gastric carcinoma cell line, SGC7901, BGC823, MKN28 and MKN45, select the highest expression cell line for next experiment. Design and synthesis RNA interference sequence for EGFL7, establish a stable transfected gastric carcinoma cell line, use this cell line and HUVEC do co-culture in vitro, detect the change in HUVEC's proliferation, adhesion and migration ability.Result:1.The 4 gastric carcinoma cell line SGC7901, BGC823, MKN45, MKN28 all express EGFL7 protein and the protein locate in the cytoplasm. Western blot show the BGC823 cell line has the highest expression; 2.The BGC823 cell line which is stable transfected (named BGC2-13), BGC823 cell line which is untranfected and BGC823 cell line which is transfected negative plasmid(named BGCHK)do co-culture with HUVEC, we find that lower EGFL7 expression cause the proliferation rate of HUVEC lower, and lower its adhesion and migration ability also (p<0.05)Conclusion:Tumor-derived can progress HUVEC's proliferation, adhesion and migration ability, and then would progress angiogenesis.