Study On The Pathogenicity Of Ureaplasma Urealyticum Serotpye 1 And 3 With The Same Concentration In Balb/c Murine Model

Objective:To study the pathogenicity of Ureaplasma urealyticum serotype1(Uu1) and 3(Uu3) with the same concentration in murine model.Method:156 oestradiol-treated adult female mice of strain Balb/c were randomly divided into 4 groups, including group A, B, C and D.Ureaplasma urealyticum serotype 1,3, 1and3, with the same concentration of 106copy/g, groupA was inoculated intravaginally with Ureaplasma urealyticum serotype 1, groupB serotype3 and groupC serotype land 3, while 12 mice of control group (groupD) inoculated sterile medium.They were necropsied at 1,3,7,14,21,35days of postinoculation randomly, to detect Uu from cervical secretions by fluorescent quantitative polymerase chain reaction (FQ-PCR), observing tissues of cervix, endometria, fallopian tube by light microscope and cervical mucosa by scanning electron microscope.Result:(1)The total positive rates of Uu in three experimental groups does not show a significant difference(P=0.273>0.05), which were 37.5%(18/48),47.9%(23/48),54.2%(26/48). And the positive rate descended as the days of postinoculation lasting. The highest positive rates of three experimental groups were 75.0%(6/8),87.5%(7/8), 87.5%(7/8)at Id after inoculation, but the conlisation didn't persist at 35d. All mice of control group were negtive. (2)There was not statistical difference in the quantity of Uu among experimental groups(P>0.05). Their peak level were2.82×106copy/g,3.55×106copy/g,6.46×106copy/g reached at 1d, but the FQ-PCR values reduced as the days of postinoculation lasting. (3)The total pathogenicity rates of three groups were significant different at 3-35d, which were35.0%(14/40),47.5% (19/40),62.5%(25/40)(P=0.048<0.05). But there were not statistical difference among experimental groups, at each time point. None of control group was positive.Conclusion:(1)In Balb/c murine model, the pathogenicity of each experimental group was shown when inoculating Uu with FQ-PCR value is 106copy/g.(2)In Balb/c murine model, the infectivity and quantatitive value were not significant different among experimental groups, but the pathogenicity of the mixed group (groupC) was the highest and Uul group(groupA) the lowest when inoculating Uu with the same concentration.