Study On Content Of Three Effective Components From Sorbus Tianschanica Rupr

Sorbus tianschanica Rupr. is a medicinal plant. Recently, studies mainly focused on pharmacological effects and seedling cultivations, which lack on effective components. This paper was study on content of three effective components from Sorbus tianschanica Rupr., which will provide theory evidence and reference for the development of pharmacodynamica and the application of this resources.The method of organic solvent extraction was used to research effective components of the whole Sorbus tianschanica Rupr.. The results showed that there are many kinds of effective components in the Sorbus Tianschanica's every nutritious organs, including carbohydrate, flavonoid, alkaloid, amino acid, protein, phenol, organic acid, cardiac glycosides, volatile oil and steroid.Spectrophotometric method was proposed for the determination of three effective components in different parts of Sorbus tianschanica. The results indicated that different effective components in the plant has different save manner. The content of totle flavonoid: fruits 6.34%, stems 4.87%, leaves 4.43%, roots 3.93%; the content of totle alkaloid: stems 0.81%, roots 0.43%, leaves 0.24%, fruits 0.21%; the content of polysaccharides: fruits 4.63%, leaves 2.06%, stems 1.00%, roots 0.89%.The optimum extraction condition of polysaccharides in fruits by ultrasonic waves was investigated by single factor experiment and orthogonal experiment. The results showed that ratio of solid to liquid, ultrasonic time and ultrasonic power were determined as main factors which affect the content of polysaccharides. The optimized conditions: ethanol concentration is 95%, ratio of solid to liquid is 1:45, ultrasonic time is 45min, ultrasonic power is 240W. In these conditions, the content of polysaccharides is 5.94%. Ratio of solid to liquid has an obviously effect.Polysaccharides in fruits was extracted by ultrasonic waves on the optimum extraction condition, and then getting rid of protein and washing with alcohol. PST1-PST4 were got after dealing with by DEAE-52 column.The results showed that the content of acid polysaccharides from the crude polysaccharides is 50.26%, is 2.99% from the Sorbus tianschanica fruits. The content of PST3 is 1.37% which is the most, the other are all 0.55% up and down. UV Spectrum and IR Spectrum were used to analyse structures. The results indicated that PST1-PST4 are all acid polysaccharides without protein, colour and other substance. And maybe the PST2 is a pure polysaccharide.