Expression Of COX-2 And VEGF In Benign Prostatic Hyperplasia Combined With Prostatitis

Objective: To study the expressions of Vascular endothelial growth factor (VEGF) and cyclooxygenase-2 in benign prostatic hyperplasia combined with Prostatitis ,analyze their correlation and to investigate the effect and molecular mechanism of prostatitis on BPH .Material and methods:60 specimens were obtained from the benign prostatic hyperplasia (BPH) patients undergoing transurethral prostatic resection(TURP).The paraffin section of the specimens were stained with hematoxylin and eosin,and observed under light microscope to examine the inflammation pathological changes. Depending on whether the benign prostatic hyperplasia combined with prostatitis, those specimens were divided into group of simple benign prostatic hyperplasia ( A group) and group of benign prostatic hyperplasia combined with prostatitis (B group), using immunohistochemistry and Real-time fluorescence quantitative PCR to detect the expressions of COX-2 and VEGF in prostate tissues.Results: (1) immunohistochemical staining: The average light density of COX-2 ,in the A group: 0.3522±0.0141. In the B group: 0.3733±0.0208, statistics result : t=85.31 P<0.05; The average light density of VEGF, in the A group: 0.4308±0.0138. In the B group: 0.4503±0.0239, statistics result : t=91.58 P<0.05; COX - 2 and VEGF expression in the B group of organizations had a positive correlation (R = 0.90 P <0.05) (2) COX-2mRNA in the A group, the relative ratio: 0.141±0.019,in the B group, the relative ratio: 0.161±0.013,statistics result : t=23.69 P<0.05;VEGFmRNA in the A group relative ratio: 0.254±0.015, in the B group, the relative ratio: 0.341±0.012, statistics result : t=58.29 P<0.05; COX - 2mRNA and VEGFmRNA expression in the B group of organizations had a positive correlation (R = 0.834 P<0.05)Conclusion: The inflammatory factor COX-2 and proliferative factor VEGF in the tissues of benign prostatic hyperplasia combined with prostatitis are significantly increased and the expression levels of both positive correlation, COX-2 by the way of up-regulating VEGF expression may be involved in the development process of benign prostatic hyperplasia.