Association Of Platelet Endothelial Cell Adhesion Molecule-1 Polymorphism With Ischemic Stroke

Ischemic stoke is one of three diseases leading to death.It is considered a variety of factors leading to ischemic stroke. Genetic factors play a decisive role in the susceptibility of ischemic stroke. The study of potential impacts of gene polymorphism and gene mutation to the incidence of ischemic stroke is a hot spots. Through the study of gene polymorphism,it will help to understand the molecular level of pathogenesis of ischemic stroke, which is expected to open up a completely new way in ischemic stroke therapy at molecular level. Atherosclerosis is the basis etiology of ischemic stroke, is a key point in the occurrence and development of atherosclerosis disease,the beginning link of inflammatory reaction is circulating inflammatory cells adhesion in endothelial cell and migration extravascular. Platelet endothelial cell adhesion molecule-1 (PECAM-1) is a necessary condition for the adhesion of leukocytes to transport the endothelium and transmigrate to foreign vessels.The aim of this study is to pinpoint whether single nucleotide polymorphisms(SNPs) in the genes coding Leu125Val and Asn563Ser for PECAM-1 are associated with CIS susceptibility in northern population of China(liaoning area).Methods1.169 subjects with CIS and 186 controls are enrolled in the study. Determination the clinical indication associated with CIS including blood fat,blood glucose,high blood pressure and BMI.2.Subjects fast 12 hours later, the patients are admitted the next morning, volunteers are on the examination day, collecting peripheral venous blood 2ml, with EDTA anticoagulant.3. Using DNA extraction kit to extract genomic DNA, and conducting DNA quantification and purity analysis.4. Primers are designed by premier5.0, Preparate primers by biotech companies.5.PEC AM-1 genotype was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Direct sequencing of PCR product to verify PCR-RFLP results. This step is completed by Shanghai Public Health Service Co., Ltd. bio-engineering techniques.6. Comparison the relationship between the two groups of genotype and allele frequency distribution with ischemic stroke.Results1.Cerebral infarction group PEC AM-1 L125 V locus LL, LV, VV genotype frequencies are as follows:17.2%,51.5%,31.3%,in the control group are 23.1%, 56.5%,20.4%, genotype frequencies consistent with Hardy-weinberg balance. Cerebral infarction group and the control group genotype distribution of PECAM-1 LI25V compared with a statistically significant difference (X2= 6.082, P= 0.048). Cerebral infarction group PECAM-1 S125A locusAA, AS,SS genotype frequencies were as follows:29.0%,51.5%,19.5%,in the control group were 23.7%,53.2%, 23.1%,genotype frequencies consistent with Hardy-weinberg balance.There is less statistically significant between case group and control group (X2=1.258, p=0.262).2. Cerebral infarction group PECAM-1 L125V V-allele frequency is 57.1%, control group 48.7%, V allele in the cerebral infarction group and the control group were significant differences in the distribution of (X2= 5.066, P= 0.024). Cerebral infarction group PECAM-1 S563A A-allele frequency is 54.7%, control group 48.7%, There is also less statistically significant between case group and control group (X2=1.258, p=0.262)3. Logistic regression analysis shows that PECAM-1 L125V polymorphic VV-based type is a result of atherosclerosis independent risk factor for cerebral infarction (OR=2.467,95%CI:1.518-4.009, P=0.000).ConclusionPECAM-1 gene Leu125Val locus gene polymorphism and ischemic stroke-related, VV genotype may be genetic susceptibility factor of ischemic stroke in Chinese population...