| BackgroundCoronary heart disease is one of the most important diseases that threat the population's health. Researchers have found that there is difference between male and female for many years, the male have higher morbidity than female before menopause period. Recently, a number of basic study and clinic invests suggested that adhesion molecules present an important role contributed to cardiovascular diseases and the pathological athroscelerosis. The vascular endothelial lesion and thrombosis effect mediated by Platelet endothelial cell adhesion molecule-1 were one of the most important mechanisms of coronary heart diseases. Several polymorphisms of platelet endothelial cell adhesion molecule-1 (PECAM-1) has been reported including three missense polymorphisms: Leul25Val in exon 3, Ser563Asn in exon 8, Gly670Arg in exon 12, and the ammo acid they expressed situated in extra cellular immunoglobulin domain or cytoplasmic domain. It is reported that the polymorphism of PECAM-1 gene was associated with coronary heart disease and the plasma levels of soluble PECAM-1 was significantly elevated in the AMI patients. These findings suggest the polymorphism of PECAM-1 gene to be involved in the development of atherosclerosis of the coronary artery by mediated the plasma concentration and functions of PECAM-1. In addition, can sex hormone mediate the plasma level of PECAM-1, and may the mediation be one of the cardiovascular protective mechanism of sex hormone. In one word, we think it is important to explore the relation of the PECAM-1 gene polymorphism with coronary heart disease, and the mediation of it and sex hormone in the plasma concentration of PECAM-1. Objective:To investigate the relationship between PECAM-1 gene and coronary heart disease. Methods:1. Clinic index of blood pressure ,body mass index, fasting blood glucose, serum cholesterol and triglyceride were measured in 156 patients with CHD and 75 controls , who were angiographically documented.2. Samples were collected when the patients were admitted to hospital. The concentration of testosterone, estrogen, progesterone was obtained by Chemiluminescence's Immunoassay and the soluble PECAM-1 was checked by ELISA.3. By polymerase chain reaction-restriction fragment length polymor-phism(PCR-RFLP), we analyzed PECAM-1 gene polymorphism in patients and controls and compared the distribution of genotype and allele in two groups. Based on this, we compared the other results and clinic index including prevalence rate of hypertension and diabetes mellitus, BMI, level of serum cholesterol and sex hormone.Results:1. The component of female, history of cigarette and alcohol, high density lipoprotein cholesterol, the morbidity of hypertension and diabetes mellitus in CHD groups were significantly higher than those of control while other clinic features such as age, total cholesterol, triglyceride and BMI showed no difference between CHD groups and controls.2. The analysis of individual genotypes of PECAM-1 gene 373G/C, 1688A/G showed significantly different distributions of genotype and allele between CHD and controls.3. The concentrations of plasma soluble PECAM-1 is significantly different between CHD and controls..4. The levels of plasma soluble PECAM-1 were significantly different among three genotype of PECAM-1 gene 373G/C.5. The levels of plasma soluble PECAM-1 were significantly different among three genotype of PECAM-1 gene 1688A/G.6. The levels of plasma soluble PECAM-1 have a negative correlation with the levels of testosterone and estrogen in male; on the contrary onlyhaving a negative correlation with estrogen in female and having nocorrelation with progesterone in both male and female.Conclusion:The polymorphism of PECAM-1 gene was associated with CHD, the genotype of Val125Val and Asn563Asn may be a genetic risk factor for coronary heart disease. The PECAM-1 gene affect the development of CHD by mediating the concentration and function... |
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