The Expression And Significance Of SIRT1 In The Articular Cartilage Of Osteoarthritis

Objective This study is undertaken to approach the the correlation and possible involvement of SIRT1 in pathogenesis and progression of osteoarthritis(OA) by detecting the expression of SIRT1 in different impaired cartilage of human OA.Methods collect 39 cartilage specimens from OA (30) and normal joints (9).49 cartilage tissues with the form of 1.0 cm×1.0 cm×0.5cm were cut from these specimens according to the Outerbridge classification.All the cartilage tissues included normal and impaired cartilage ones.Then All the samples were ascribed to four groups of normal(A),minor(B),moderate(C) and severe(D) on the basis of the improved grading system of Mankin. The expression of SIRT1 were detected in these samples by the immunohistochemistry (SABC), and then the statistical data of expression was analysed by SPSS 17.0.Results1.SIRT1 was detected in the tissues of all the four groups.The amount of average gray level of SIRT1 expression respectively was (Group A):104.14±8.30, (Group B):125.94±10.70, (Group C):133.22±9.62, (Group D):163.07±9.78.The mutual comparisons of all these groups have statistical significance except the one between Group B and Group C (P>0.05).2. The average gray level of SIRT1 increases with the improving grade of the improved grading system of Mankin,(r=0.893,P<0.01). That indecates the expression of SIRT1 correlates with the degenerate level of cartilage negatively.Conclusions1. The down-expression of SIRT1 may induce the pathogenesis and progression of osteoarthritis(OA).2. The expression of SIRT1 correlated with the degenerate level of cartilage negatively. SIRT1 may become the monitoring indicator of the development of osteoarthritis(OA).