Studies On The In Vitro Anti-cancer Effects Of Centipede Extract On Breast Cancer Cell Line MDA-MB-231

Objective:To investigate sensitivity of the effect of centipede extract on breast cancer cell lines and provide evidences for further clinic therapy of breast cancerMethods:Cultured breast cancer cells MDA-MB-231 are intervened with different concentration of centipede extract. MTT assay and concentration-time curve are used to study the effect sensitivity of centipede extract on breast cancer cell lines; Inverted light microscope and electron microscope are used to observe cell changes in morphology and ultrastructure.Results:①The results of MTT assay showed that different concentrations of centipede extract all can inhibit the growth of human breast cancer cells MDA-MB-231.The inhibition rates were (71.26±2.64)%, (62.48±6.67)%, (56.26±3.17)%, (48.63±4.48)%, (42.13±4.73)%, (34.5±2.98)% and (26.89±2.95)% respectively,while the Corresponding concentration of centipede extract were 80mg/ml,40 mg/ ml,20 mg/ml, 10mg/ml,5 mg/ml,2.5 mg/ml and 1.25 mg/ml,and each group had significant differences(P<0.01). ECP concentrations in 80mg/ml inhibition rate of pirarubicin (THP) 10μg/ml was no significant difference in inhibition rate(P>0.05), roughly the same, ECP in the inhibition of 20mg/ml and 40mg/ml group rate and THP5μg/ml no significant difference in inhibition rate (P>0.05), roughly the same. IC50 of centipede extract to MDA-MB-231 was 12.5mg/ml.②Drug concentration-time curve revealed that the viable cell counts of MDA-MB-231 has direct relations with the concentration and administrative time of centipede extract. When treated with 12.5mg/ml and 25mg/ml centipede extract, viable counts of MDA-MB-231 sharply decrease after 24 hours treatment and the difference among treatment groups is significant (P<0.01).③After 48hr intervention, there were no inhibitory effect observed by light microscopy in groups with concentration less than 10mg/ml; viable counts of MDA-MB-231 decreased and the cells transformed to round and small when concentration were more than 10mg/ml; With treated with 80mg/ml, large number of necrotic cells and irregular cell debris were seemed. Meanwhile, margination and condensation of chromatin, fragmentation and condensation of nucleus and formation of apoptotic bodies were found in cells by electron microscope which had higher density.Conclusion:①Extract of centipede can inhibit the growing of human breast cancer cells MDA-MB-231 and the effect was concentration-time dependent;②Extract of centipede can induce MDA-MB-231 cell line to apoptosis in low concentration and directly kill it in middle or high concentration.