Effects Of Resveratrol Supplementation On The Repair Of Exercise-induced Skeletal Muscle Micro- Damage Of Mouse

Resveratrol is a phytoalexin produced by some plants when pathogens invade or environments worsen. Resveratrol is found in a wide variety of dietary sources including grapes, plums, peanuts, and other food products. The present study showed that resveratrol had a wide-array of biological activities, such as scavenging free radicals, effect on anti-lipid peroxidation, adjustment to Ca2+, also function to phytoestrogen etc. At present, resveratrol is concentrated mainly in the field of medicine and Pharmacology, in sports medicine is still rare. This study investigated the supplement resveratrol to promote exercise-induced skeletal muscle micro-damage repair effects and possible mechanisms, for the prevention and treatment EIMmD, and develop a new sports nutrition supplements provides theoretical and experimental basis.Methods: 70 male Kunming mice were randomly divided into three groups: control group (CG), exercise group (EG) and exercise + resveratrol group (ER), and EG group and ER group was divided into three sub-groups by post-exercise 0 hour,24 hours,48 hours, which every group has 10 mice. The mice of ER were given resveratrol with 10 mg/kg/d, and taken protocol of treadmill training for 4-week, then had a gradient of 0 and a speed of 18m/min, continued 90min of treadmill exercise as did the mice of EG. Mice were slay respectively. The histological change of myofibril were observed by Electron microscope. The activity of serum CK and LDH , muscle SOD and MDA, concentration of muscle mitochondria Ca2+ , and muscle GUS were measured using Ultraviolet Spectrometer.Results:(1) The electron microscope results showed that EG appeared sarcomere disarray, Z-line streaming, Z-line waveform changes, the injury level of ER was less than EG, and post-exercise 48h the injury in skeletal muscle have been restored.(2) Compared with CG, the activity of serum CK and LDH was significantly increased in EG, especially post-exercise 24h(P<0.05). The CK and LDH of ER serum was significantly lower than that of EG, especially post-exercise 24h, 48h(P<0.05).(3) The activity of SOD was significantly decreased in EG compared with CG , and the MDA was significantly increased in EG compare with CG, especially post-exercise 24h(P<0.05).The SOD of ER was significantly increased than that of EG, and the MDA of ER was significantly lower compared with EG(P<0.05).(4) Compare with CG, the concentration of mitochondria Ca2+ was significantly increased in EG, especially post-exercise 24h(P<0.05). The concentration of mitochondria Ca2+ of ER was significantly lower than that of EG(P<0.05).(5) Than CG , the content of muscle GUS was significantly increased in EG(P<0.05). There were no significantly difference of GUS between ER and EG(P>0.05), but post-exercise 48h was significantly lower than post-exercise 0 hour group(P<0.05).Conclusion:(1) After the long time treadmill exercise, the mice skeletal muscle presents the obvious damage change, Supplemented resveratrol may suppress skeletal muscle ultrastructure damage change by exercise.(2) Supplement resveratrol can improve mice antioxidant capacity, lower lipid peroxidation, regulation of muscle mitochondrial Ca2+ concentration, maintaining skeletal muscle cell structure and function of stability, and thereby to promote the role of EIMmD repair.