Objective:To study the trend of autonomic nervous in three pairs of suporting ligaments of cervix uteri.Methods:Cut off the two sides of cardinal ligaments(CL), uterosacral ligaments(USL) and vesicouterine ligaments(VUL) in three fixed adult female cadavers. Immunohisto -chemical analysis of surgical margins was performed to study the distribution of autonomic nerves using a general nerve marker (S100 Protein) within sections of biopsies.Results:(1)The mean length of cardinal ligaments(CL) was 5.0cm, autonomic nervous located in the middle part of the ligaments at a distance of 4.5cm from cervix uteri,in the middle and inferior part of the ligaments and closed to the ventral side at 3.0cm and 2.0cm,in the caudal and ventral side of the ligaments at 0.5cm.(2)The mean length of uterosacral ligaments (USL) was 6.5cm, autonomic nervous located in the medial and top part of the ligaments at a distance of 6.0cm from cervix uteri,in the middle and inferior part of the ligaments and closed to the lateral side at 4.5cm, 3.0cm and 1.5cm,in the caudal and lateral side of the ligaments at 0.5cm(.3)The mean length of vesicouterine ligaments(VUL) was 2.3cm, autonomic nervous located in the medial side of the vesical veins in deep layer of the vesicouterine ligaments at a distance of 2.0cm, 1.5cm,1.0cm and 0.5cm from cervix uteri.Conclusion: Autonomic nervous started in the middle part of cardinal ligaments(CL) and went medially,downward and forward from pelvic wall to cervix uteri, went laterally, downward and forward from pelvic wall to cervix uteri in uterosacral ligaments(USL) and located in the medial side of the vesical veins in deep layer of the vesicouterine ligaments (VUL).
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