| Objective:To investigate the effect of bone marrow stem cells mobilization on treatment of acute myocardial infarction in rats.Methods: To observe the effect of granulocyte colony stimulating factor(G-CSF)mobilized bone marrow stem cells on acute myocardial infarction animals.90 SD rats were selected to make myocardial infarction model by opening chest and ligating the left anterior descending coronary artery. These rats were randomly divided into 3 groups: A is the double phasic G-CSF mobilization group: the first phase was injected the normal saline diluted G-CSF about 40μg/kg/d 3 hours later of the model completed, continuous 5 days (No. 0-4d), the second phase was injected G-CSF for 5 days continuously began from the 7th day (No. 7-11d); B is the single phasic G-CSF mobilization group: 3 hours after the model completed, the rats were only injected the same dose G-CSF for 5 days continuously; C is the control group: 3 hours after the model completed, the rats were only injected the same dose normal saline for 5 days continuously. All the rats were received intraperitoneal injection of accumulated 5-bromo-2-deoxy uridine (BrdU) from two days before surgery to five days after surgery. Blood samples were collected from the rats of the 3 groups at different time points to detect the trend of CD34+/WBC by flow cytometry and the changes of serum vascular endothelial growth factor (VEGF) by enzyme-linked immunosorbent assay (ELISA). After tested cardiac function using Cardiac ultrasound at different time points, myocardial tissue samples were measured myocardial infarct size by triphenyl tetrazolium chloride (TTC) staining method, observed the pathological changes by hematoxylin-eosin (HE) staining method, detected the expression of CD34+ cells, BrdU-positive cells and theⅧfactor in the infarcted zone by immunohistochemical, detected the apoptosis by TdT-mediated dUTP nick end labeling (TUNEL). Results: The peripheral blood CD34+/WBC ratio of two-phase G-CSF bone marrow stem cell mobilization myocardial infarction rats was increased significantly, up to 0.508±0.030%, maintain 4 to 7 times of the normal level about a week; the expression level of the serum VEGF increased up to 20.23±0.51 pg/ml, compared with other groups, the difference was significant statistically (P<0.05). After 28 days of the two-phase mobilization, the left ventricular ejection fraction was 68.96±5.79%, compared with the single-phase group 52.80±3.21% and the control group 40.22±3.79% were improved significantly. The size of the myocardial infarction, the degree of pathological damage and the apoptotic cells were decreased, the high expression of the CD34+ cells, BrdU-positive cells and angiogenesis of the myocardial infarction area and surrounding area, the difference was significant statistically (P<0.05).Conclusions:①The double phasic G-CSF mobilized is better than the single phasic group;②Two-phase bone marrow stem cell mobilization can promote the expression of serum vascular endothelial growth factor and the angiogenesis of myocardial infarction and surrounding areas effectively;③Two-phase bone marrow stem cell mobilization can improve cardiac function after myocardial infarction through reduce apoptosis and myocardial injury.
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