| Relationship between the content of serum TNF-αand the expression of TNF-αin perihematomal tissue and apoptosis in patients with hypertensive intracerebral hemorrhageObjective To investigate the relationship between the content of serum TNF-αand the expression of TNF-αin perihematomal brain tissue and apoptosis in patients with hypertensive intracerebral hemorrhage.Methods Thirty patients with hypertensive intracerebral hemorrhage(HICH) were randomly divided into<6 h,6-12 h,12-24 h,24-48 h,48-72 h and> 72h groups according to the time from symptom onset to surgery(n=5 in each group).There were no significant differences among the basic clinical situation,amount of bleeding, bleeding sites,preoperative Glasgow coma scale scores and operation duration in all groups.A double-antbody sandwich enzyme-linked immunosorbent assay was used to determine the content of serum TNF-αin perihematomal tissue.Immunohistochemistry was used to detect the expression of TNF-αin perihematomal tissue,and TUNEL staining was used to detect the apoptosis cells.Results The numbers of TNF-αpositive cells and the content of serum TNF-αin perihematomal tissue reached the peaks at 12-24 h and 24-48 h respectively after intracerebral hemorrhage,respectively. There was a positive correlation between them (r=0.924, P<0.05). The numbers of apoptosis cells in perihematomal tissue reached peak at 24-48h. There was a positive correlation between the numbers of apoptosis cells and the numbers of TNF-αpositive cells in perihemvenatoma (r= 0.871, P<0.05).Conclusion The content of serum TNF-α, the numbers of TNF-αpositive cells and apoptosis cells in perihemvenatomal tissue were increased significantly. The numbers of TNF-αpositive cells in perihemvenatomal tissue were positive correlated with The content of serum TNF-αand he numbers of apoptosis cells in perihemvenatomal tissue. TNF-αmay be related to apoptosis.
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