The Lung Protective Effects Of Sivelestat On Oleic Acid-Endotoxin Mediate ALI/ARDS In Rats

Objective To investigate the protective effects of the sivelestat injection on the lung in rats with ALI/ARDS by sequencely injected oleic acid and endotoxin.Methods ALI/ARDS model was reproduced in the rats by sequencely injectioning the oleic acid and endotoxin. Fourty-eight heathy adult Wistar rats were randomly divided into four groups:control group, model control group, Pre-treatment group, Reprocessing group.Animals in each group were sacrificed after 6h modeling and separation of lung tissue weighing, calculation of lung tissue wet and dry ratio of pulmonary pathology observed in bronchoalveolar lavage fluid neutrophil count.Serum levels of neutrophil elastase(NE) and serum levels of TREM-1 was determined by enzyme linked immunoadsorbent assay(ELISA),The BALF of neutrophil elastase(NE) was determined by enzyme linked immunoadsorbent assay(ELISA) also.Light microscopy was used assess the pathological changes in lung and account the neutrophilic granulocyte in each BALF.Results Compared with control model group,The neutrophil elastase levels in serum and bronchoalveolar lavage fluid(BALF) of was significantly decreased at 6h in Instantly treatment group and later treatment group,(p<0.01),and Serum levels of TREM-1 was significantly also decreased,(p<0.01).the counts of neutrophilic granulocytes of bronchoalveolar lavage fluid(BALF) and the lung wet/dry weights apparently descend,(p<0.01).Compared with later treatment group,The neutrophil elastase levels in serum and bronchoalveolar lavage fluid of was significantly decreased at 6h in Instantly treatment group,(p<0.05),and Serum levels of TREM-1 was significantly also decreased,(p<0.05).the counts of neutrophilic granulocytes of bronchoalveolar lavage fluid and the lung wet/dry weights apparently descend,(p<0.05).Conclusions neutrophil elastase-sivelestat was definitely protective effects on the lung in rats with ALI/ARDS,especial the instantly treatment.