Basic And Clinical Research Of Changes Of Immunocytes And Cytokines In Patient With HBV Infected Liver Disease

Objective To explore the relationship of changes between immunocytes (including CD³⁺ cells, CD⁴⁺ cells, CD⁸⁺ cells and NK cells) and cytokines (MIF, IL-17 and IL-10) in peripheral blood of HBV infected liver disease, and explore the possible role and clinical significance of the pathogenesis in patients with HBV-infected liver.Method In the first experimental group selected 48 patients with chronic hepatitis B (HBeAg negative and positive 24 cases), 81 cases of hepatitis B liver cirrhosis (Child-Pugh classification A grade 23 cases, B grade 32 cases, C grade 26 cases), 40 cases of primary liver cancer. 45 cases of HBV-DNA negative patients and 36 patients with HBV-DNA positive as the experimental group, 26 healthy people as control group, using flow cytometry method to measure content of immune cells, including T lymphocyte subsets (CD³⁺ cells, CD⁴⁺ cells, CD⁸⁺ cells) and NK cells; using PCR method to measure content of HBV-DNA .In the second experimental group to selecte from the first group 24 patients with chronic hepatitis B (HBeAg negative and positive, 12 cases), 24 cases of hepatitis B patients with decompensated cirrhosis and 24 cases of primary liver cancer as the experimental group, 24 healthy people as control group, using enzyme-linked immunosorbent assay (ELISA) method to measure content of MIF, IL-17 and IL-10 in serum.Results In the first experimental group the contents of CD³⁺ cells, CD⁴⁺ cells and NK cells in peripheral blood of HBV infected liver disease were obviously lower than that in control group, and CD⁸⁺ cells was increased(P<0.05).However, in HBeAg-negative and positive group of chronic hepatitis B there was no significant difference(P> 0.05). The contents of CD³⁺ cells, CD⁴⁺ cells and NK cells were decreased gradually along with increasing of Child-Pugh classification liver cirrhosis group, and CD⁸⁺ cells content was increased(P <0.05), there is statistical significance. Changes in liver cancer patients was also similar. HBV-DNA patients between positive and negative there is no significant change in T cell subsets ,but compared with the control group , there is statistical significant.In the second experimental group MIF and IL-17 were significantly increased, IL-10 was significantly decreased, compared with the control group (P<0.05) , there is significant difference. But in chronic hepatitis B, cirrhosis and liver cancer group MIF, IL-17 and IL-10 levels were no significant difference(P> 0.05).Conclusions T lymphocyte subgroup, NK cell ,MIF, IL-17 and IL-10 may be involved in the pathogenesis of HBV-infected liver disease, and increase changes tissue pathological in liver ,the measure of lymphocyte and three cytokines has important clinical significance to judging of condition and prognosis HBV- infected liver disease.