Study On Myocardial Energy Metabolism And Mitochondrial Function Changes In I/R

Objective: 1. to establish in vitro ischemia / reperfusion injury model in rats to monitor changes in cardiac function and biochemical indicators of myocardial;Verify the in vitro ischemia / reperfusion injury model was established successfully. 2. Successfully established ischemia / reperfusion injury model; then test the mitochondrial function and energy metabolism.Methods: 1. SD rats were killed off the spine, removed the heart quickly into 4℃KH solution, aortic cannulation, silk ligation fixed, anchored in the Langendorff perfusion apparatus, connecting the pressure transducer sensor, start perfusion; RM-6280 multi-channel recording and analysis system recorded the physiological analysis of cardiac function; collecting fluid after perfusion and testing the myocardial biochemical indicators. 2. The experimental animals were divided into normal control group, ischemia-reperfusion group and ischemia group (n = 8). After perfusion in each group, taking the left ventricular myocardial to extract the protein of AMPK and Cyt - C. using Western blot to determine the protein of AMPKαand Cyt - C.Results: 1.The results In vitro ischemia / reperfusion injury model: Compared with the control group, LVSP (P <0.05), + dp / dtmax (P <0.05) and -dp/dtmax (P <0.05 ) decreased in experimental group; LVEDP (P <0.05) increased in experimental group with a significant difference; and HR groups was not significant (P > 0.05). Compared with the experimental group, I / R myocardial biochemical indicators CK, LDH, CK-MB was significantly higher (P <0.05). We can say the in vitro ischemia-reperfusion model is established successfully. 2. The expression of AMPKαprotein was significantly decreased (P <0.05) in Ischemia-reperfusion group. The expression of cytochrome C protein were significantly higher (P <0.05). Conclusions: 1. the in vitro ischemia / reperfusion injury model was successfully established. 2. The expression of AMPKαprotein was significantly decreased (P <0.05) in Ischemia-reperfusion group, but the expression of cytochrome C protein was significantly increased.