| Objective: To investigate the effect and mechanism of serum endotoxin on heart during liver I/R injury. Methods: A model of liver I/R injury was established by imitation. 56healthy Wistar male rats were randomly divided into 7 groups as following(n=8):the control group(CTL), simply ischemia 30min without reperfusion (I group), reperfusion following ischemia 30min (I/R group), 2 hours reperfusion following ischemia 30min (I/R2h group), 4 hours reperfusion following ischemia 30min (I/R4h group), 6 hours reperfusion following ischemia 30min (I/R6h group) and pretreatment group (654-2 group). The cardiac function were measured by BL-420 E~+data acquisition and analysis system. The level of serum endotoxin was measured. The level of insulin and insulin antibody in heart were measured by radioimmunoassay. The changes of MDA, MPO and lactic acid in heart were measured by kit. Results: HR decreased obviously in the I group and the I/R group, but the amount increase gradually with the prolongation of reperfusion, there was a statistic significance in I/R2h group, I/R4h group, I/R6h group compared with I/R group(P<0.05), there was no statistic significance compared with CTL(P>0.05), there was no statistic significance in 654-2 group compared with CTL and I/R4h group(P >0.05). The decrease of LVSP has statistic significance in I group and I/R group compared with CTL(P<0.05), the level of LVSP rise to normal in I/R2h group, the decrease of LVSP has statistic significance in I/R4h group and I/R6h group compared with CTL(P<0.05), there was a statistic significance in 654-2 group compared with CTL and I/R4h group(P<0.05). The increase of LVEDP has statistic significance in I/R4h group compared with I group(P<0.05), there was a statistic significance between I/R6h group and the other groups (P<0.05), the decrease of LVEDP has statistic significance in 654-2 group compared with CTL and I/R4h group (P<0.05). Although the level of +dp/dtmax fluctuated in the different group, the decrease of +dp/dtmax has statistic significance in I/R6h group compared with CTL, I group, I/R group and I/R2h group(P< 0.05), there was a statistic significance in 654-2 group compared with CTL and I/R4h group(P<0.05). The rising of-dp/dtmax has statistic significance in I/R group and I/R6h group compared with CTL(P<0.05), there was a statistic significance in 654-2 group compared with CTL and I/R4h group (P<0.05). The increase of endotoxin has obviously statistic significance in CTL compared with the other groups (P<0.05), the level of endotoxin reach to peak in I group and I/R group, the amount decrease gradually with the prolongation of reperfusion, but still higher than CTL(P<0.05), there was a statistic significance in 654-2 group compared with CTL and I/R4h group(P<0.05). From I group, the increase of MDA in cardiac organization has statistic significance in CTL compared with the other groups(P<0.05), there was a statistic significance in I/R2h group, I/R4 group, I/R6h group compared with I group and I/R group(P<0.05), there was a statistic significance in 654-2 group compared with CTL and I/R4h group(P<0.05). The level of MPO obviously increased in every reperfusion group, there was a statistic significance compared with CTL and I group(P<0.05), but there was no statistic significance among them(P>0.05), there was a statistic significance in 654-2 group compared with CTL and I/R4h group(P<0.05). The change of lactic acid is that the rising of lactic acid has statistic significance in I/R2h group, I/R4h group, I/R6h group compared with CTL, I group, I/R group(P<0.05), there was no statistic significance in 654-2 group compared with CTL(P>0.05), but the decrease of lactic acid in 654-2 group has a statistic significance compared with I/R4h group(P<0.05). The decrease of insulin in cardiac organization was statistic significance in I/R group compared with I group(P<0.05), however, the level of insulin increased obviously in I/R2h group, there was a statistic significance compared with I/R group(P<0.05), there was a statistic significance in I/R4h group and I/R6h group compared with CTL and I group and I/R2h group(P<0.05), there was no statistic significance in 654-2 group compared with CTL (P>0.05), but there was a statistic significance compared with I/R4h group(P<0.05). The change of insulin antibody is that there was no statistic significance among every group(P > 0.05). Conclusion: During the process of liver I/R injury, endotoxin was absorbed from intestine and reducement of deintoxication of hepatic led to endotoxemia which plays one of the roles in the changes of the cardiac function, metabolism and structure. 654-2 plays a role in protecting the heart during ischemia reperfusion injury.
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