Study On Model Establishment Of Arterial Restenosis In Rats And Targeting Therapy Of Restenosis Of Coronary Artery By P27

Objective:To establish the rats model of carotid restenosis, and investigate the inhibition effects of p27 gene specificity driven by SM22alpha gene (Lenti-SM22alpha-p27) promoter on the proliferation of vascular smooth muscle cells(VSMCs).Methods:Injuried the carotid of rats by balloon, observed the proliferation of VSMCs in intima.Infected animals with Lenti-SM22alpha-p27, and contrast the proliferation of VSMCs to the rats injected by Taxol at the same time.Results:There were all neointima formed in balloon injuried cartiods, and would be thicker when time goes on in 28 days. the rats treated by p27 have few VSMCs proliferation and significantly different from rats cured by taxol.Conclusion:The pathophysiology appeared in balloon injuried rats was similar to in-stent restenosis (ISR), can be used for the studies of ISR. The Lenti-SM22alpha-p27 can infect VSMCs successfully and over-expressed p27 to have a significant inhibition of proliferlation to VSMCs.