Establishment And Evaluation Of A Murine Nephritis Model With Chronic Graft-versus-host Disease

Chrinic graft-versus-host d isease ( cGVHD) is a n increasingly frequent complication of allogeneic stem cell transplantation. Current therapies for cGVHD reduce symptoms but are not cures. TheDBA→/J2B 6D2f1 minor histocompatibility antigen-mismatched model, which reflects c linical a nd pa thological s ymptoms o f human cGVHD, was used in this study. The spleen,thymus,lymph nodes cells was isolated from the donor mice and transplant into the receptor by intravenous injection according a certain proportion.From t he s urvivalrate,Weight,the percentage of urine protein development, Serum levels of autoantibodies and pathobiological mechanisms we demonstrated the success o f t he model.Then the r apamycin,a c linically d rugs, w as used to further evaluate the model. The rusults show our model is very successful. So a drug screening platform was established, and the better drugs for cGVHD is possible.In this study, three subjects were investigated:(1) Take DBA/2J mice as donor, B6D2F1 mice as acceptor, isolate the spleen,thymus,lymph nodes cells from the donor and transplant into the acceptor, so the model was established..(2) Evaluate the model from the survivalrate,Weight,the percentage of urine protein development, Serum levels of autoantibodies and pathobiological mechanisms, demonstrated the succeful of the model. Then the rapamycin was used to evaluate the model, the results tell the mod′es lsuccess.(3) The screening platform for cGVHD was successfully established, so that more specific and effective drug will be getted for patients who are urgently needed. Also, the model can help us to further understand the mechanism of cGVHD.